| 林艳云,王峰,王海波,等.氮氧自由基化合物对60Co γ射线致BALB/c小鼠辐射损伤的防护作用[J].中华放射医学与防护杂志,2016,36(6):401-405.Lin Yanyun,Wang Feng,Wang Haibo,et al.Radioprotective effects of nitroxide compound NHCOCH3-TEMPO in BALB/c mice[J].Chin J Radiol Med Prot,2016,36(6):401-405 |
| 氮氧自由基化合物对60Co γ射线致BALB/c小鼠辐射损伤的防护作用 |
| Radioprotective effects of nitroxide compound NHCOCH3-TEMPO in BALB/c mice |
| 投稿时间：2015-11-09 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.06.001 |
| 中文关键词: 氮氧自由基 BALB/c小鼠 γ射线 辐射防护 |
| 英文关键词:Nitroxide compound BALB/c mice γ-rays Radiation protection |
| 基金项目:国家科技支撑计划(2013BAK03B05);国家973计划(2011CB503704);国家自然科学基金(81272490,31470828);军队指令性项目(13BJYZ29) |
| 作者 | 单位 | E-mail | | 林艳云 | 710032 西安, 第四军医大学军事预防医学系放射医学教研室 | | | 王峰 | 030006 太原, 武警山西总队医院CT室 | | | 王海波 | 710032 西安, 第四军医大学军事预防医学系化学教研室 | | | 蒋大鹏 | 710032 西安, 第四军医大学军事预防医学系放射医学教研室 | | | 高鹏 | 710032 西安, 第四军医大学军事预防医学系放射医学教研室 | | | 任正华 | 710032 西安, 第四军医大学军事预防医学系放射医学教研室 | | | 吴涛 | 710032 西安, 第四军医大学军事预防医学系放射医学教研室 | | | 郎海洋 | 710032 西安, 第四军医大学军事预防医学系放射医学教研室 | | | 曾丽华 | 710032 西安, 第四军医大学军事预防医学系放射医学教研室 | | | 郭国祯 | 710032 西安, 第四军医大学军事预防医学系放射医学教研室 | | | 丁桂荣 | 710032 西安, 第四军医大学军事预防医学系辐射生物学教研室 | dingzhao@fmmu.edu.cn |
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| 中文摘要: |
| 目的 观察氮氧自由基化合物NHCOCH3-TEMPO对雄性BALB/c小鼠电离辐射损伤的防护效果。方法 将120只雄性BALB/c小鼠按随机数字表法分成4和7 Gy照射组,每组60只,每组再按随机数字表法分成6组,每组10只,分别为生理盐水对照组、生理盐水照射组、低剂量TEMPO照射组、中剂量TEMPO照射组、高剂量TEMPO照射组和阳性对照(WR-2721)组。60Co γ射线照射前0.5 h动物腹腔注射防护药物,剂量分别为:WR-2721组200 mg/kg、低、中、高剂量TEMPO组分别为100、200和400 mg/kg、生理盐水对照组和生理盐水照射组给予等量生理盐水。4 Gy全身照射组用于观察小鼠照后8和15 d骨髓有核细胞数、骨髓DNA含量和外周血象的变化;7 Gy全身照射用于观察小鼠照后30 d生存率。结果 4 Gy照射后,TEMPO预处理组小鼠骨髓有核细胞计数、骨髓DNA含量较生理盐水照射组均明显增加(t=2.53~6.13,P<0.05);中剂量TEMPO预处理组小鼠外周血白细胞数较生理盐水照射组明显增加(t=4.34,P<0.05),但外周血红细胞数与生理盐水照射组相比,差异无统计学意义。7 Gy照射后,低剂量TEMPO预处理组小鼠30 d存活率较生理盐水照射组明显增加(χ2=5.934,P<0.05)。结论 氮氧自由基化合物NHCOCH3-TEMPO对雄性BALB/c小鼠γ射线辐射损伤有一定的防护作用。 |
| 英文摘要: |
| Objective To investigate the protective effects of nitroxide compound NHCOCH3-TEMPO against γ-ray irradiation-induced injury in male BALB/c mice. Methods 120 BALB/c male mice were randomly divided into two super group, each super group then were randomly divided into 6 groups:control group, normal saline(NS)+γ group, low-dose TEMPO+γ group, middle-dose TEMPO+γ group, high-dose TEMPO+γ group and the positive control (WR-2721)+γ group. BALB/c mice were intraperitoneally injected with nitroxide compound at 0.5 h before whole body 60Co γ-ray irradiation. The drug dosages were as follows:WR-2721 group 200 mg/kg, low-dose TEMPO group 100 mg/kg, middle-dose TEMPO group 200 mg/kg, high-dose TEMPO group 400 mg/kg. The first super group of the mice was given 7 Gy γ-ray whole-body irradiation, and then 30 d survival rate was observed. The second super group of the mice was given 4 Gy γ-ray whole-body irradiation. The number of nucleated cells and the content of DNA in bone marrow, as well as the blood routine were detected. Results At 8th and 15th day after 4 Gy γ-ray irradiation, the number of bone marrow nucleated cells, the content of DNA significantly increased in TEMPO+γ-ray groups compared with NS+γ-ray group(t=2.53-6.13,P<0.05). The number of white blood cells significantly increased in middle dose TEMPO+γ-ray groups compared with NS+γ-ray group(t=4.34,P<0.05), while there was no significant difference in the number of red blood cells between the TEMPO+γ-ray group and the NS+γ-ray group. After 7 Gy γ-ray irradiation, the survival rate of mice on the 30th day significantly increased in low-dose TEMPO+γ-ray group compared with NS+γ-ray group(χ2=5.934,P<0.05). Conclusions The nitroxide compound NHCOCH3-TEMPO could provide radio protective effects in BALB/c mice. |
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