中华放射医学与防护杂志

左晓勇,宋淑军,司少艳,等.维生素D对不同剂量X射线照射小鼠免疫功能的保护作用[J].中华放射医学与防护杂志,2016,36(4):268-271.Zuo Xiaoyong,Song Shujun,Si Shaoyan,et al.Protective effect of vitamin D on the immune function of mice in response to radiation[J].Chin J Radiol Med Prot,2016,36(4):268-271

维生素D对不同剂量X射线照射小鼠免疫功能的保护作用

Protective effect of vitamin D on the immune function of mice in response to radiation

投稿时间：2015-12-23

DOI：10.3760/cma.j.issn.0254-5098.2016.04.007

中文关键词: 维生素D 辐射免疫功能

英文关键词:Vitamin D Radiation Immune function

基金项目:

作者	单位	E-mail
左晓勇	100101 北京, 安徽医科大学解放军306临床学院
宋淑军	100101 北京, 解放军306医院特种医学实验研究中心
司少艳	100101 北京, 解放军306医院特种医学实验研究中心
王莹	100101 北京, 解放军306医院特种医学实验研究中心
单改仙	100101 北京, 解放军306医院特种医学放疗中心
任晔	100101 北京, 解放军306医院特种医学放疗中心
戴卓捷	100101 北京, 解放军306医院特种医学放疗中心
王宗烨	100101 北京, 安徽医科大学解放军306临床学院	wangzye@163.com

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中文摘要:

目的观察维生素D对不同剂量X射线照射小鼠免疫功能的影响。方法将小鼠采用随机数字表法分为5组:健康对照组、2 Gy和5 Gy照射组、2 Gy+药物(维生素D)组和5 Gy+药物(维生素D)组。给药组小鼠在不同剂量X射线照射后,连续7 d腹腔注射6 IU维生素D[1,25(OH)₂D₃]/g体重,于给药后第8天,5组小鼠均处死。测定小鼠体重、胸腺和脾脏指数,ConA诱导的脾脏T淋巴细胞增殖能力,ELISA法检测脾细胞IL-2的分泌量。结果与健康对照组相比,2和5 Gy照射组小鼠脾脏T淋巴细胞增殖能力、脾细胞IL-2的分泌量均显著降低(F=36.20、7.13,P<0.05);与2 Gy照射组相比,2 Gy+药物组小鼠胸腺指数、脾脏指数、脾脏T淋巴细胞增殖能力均显著增高(t=-2.54、-2.24、-2.84,P<0.05);与5 Gy照射组相比,5 Gy+药物组小鼠的胸腺指数、脾细胞IL-2的分泌量显著增高(t=-5.02、-2.64,P<0.05)。结论维生素D能够改善受照小鼠的免疫功能,但随照射剂量增大,改善作用减弱。

英文摘要:

Objective To investigate the effect of vitamin D on the immune function of mice irradiated with different doses of X-rays. Methods The mice were randomly divided into five groups: controls, 2 and 5 Gy irradiated groups, 2 and 5 Gy X-rays combined with vitamin D groups. Vitamin D (6 IU/g) was intraperitoneally injected into the mice after irradiation for 7 days. On day 8, all mice were killed, and body, thymus and spleen of each mouse were weighed. The thymus and spleen indexes, and proliferation of spleen T lymphocytes were assessed. The level of IL-2 in the supernatant of spleen cells was measured by ELISA. Results Compared with control group, proliferation of spleen T lymphocytes and the level of IL-2 in the supernatant significantly decreased in 2 and 5 Gy groups (F=36.20,7.13, P<0.05). Compared with 2 Gy group, the thymus index, spleen index and proliferation of spleen T lymphocytes were significantly increased in the 2 Gy plus vitamin D group (t=-2.54,-2.24,-2.84, P<0.05). Compared to the 5 Gy group, the thymus index and the level of IL-2 in the supernatant significantly increased in the 5 Gy plus vitamin D group (t=-5.02,-2.64, P<0.05). Conclusions Vitamin D could restore the immune function of mice after radiation, while the restore capacity was decreased along with the increase of radiation dose.

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