| 刘佳,高刚,吴世凯,朴春南,刘建香.放射治疗对乳腺癌患者血浆中microRNAs表达的影响[J].中华放射医学与防护杂志,2016,36(4):260-263,302 |
| 放射治疗对乳腺癌患者血浆中microRNAs表达的影响 |
| Radiotherapy induced microRNA expression in the plasma of breast cancer patients |
| 投稿时间：2016-01-06 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.04.005 |
| 中文关键词: 电离辐射 乳腺癌 放射治疗 miRNA 剂量效应 |
| 英文关键词:Ionizing radiation Breast cancer Radiotherapy miRNA Dose response |
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| 中文摘要: |
| 目的 分析乳腺癌放疗患者血浆中miR-210-3p、miR-221-3p、miR-21-5p、miR-150-5p的表达水平的变化,为寻找非均匀辐射致损伤的早期生物标记物提供实验依据。方法 选取乳腺癌患者13例,于放疗前(0 Gy)、放疗2、10、20和30 Gy后的24 h采血。采用实时荧光定量PCR(qRT-PCR)法检测受检者血浆miRNAs的表达水平。结果 不同剂量照射后miR-210-3p、miR-221-3p、miR-21-5p的表达差异无统计学意义(P>0.05)。miR-150-5p 2 Gy照射后与照射前表达水平差异无统计学意义,但随着照射剂量增加,其表达水平显著下调,分别为照射前的0.808、0.605、0.565(χ2=18.76,P<0.05)。而在人表皮生长因子受体2(HER2)、雌激素受体(ER)、孕激素受体(PR)的阴性组和阳性组中,miR-150-5p表达差异均无统计学意义(P>0.05)。结论 电离辐射可诱导miR-150表达显著下调,且存在一定的剂量依赖关系。 |
| 英文摘要: |
| Objective To investigate the expressions of miR-210-3p, miR-221-3p, miR-21-5p and miR-150-5p in the plasma of breast cancer patients before and after radiotherapy in order to establish reliable early biomarkers of non-uniform radiation injuries. Methods Blood samples were collected from 13 patients before radiotherapy (0 Gy) and 24 h after radiotherapy of 2, 10, 20, 30 Gy. The miRNAs in the blood plasma were detected with qRT-PCR. Results The levels of miR-210-3p, miR-221-3p, miR-21-5p had no significant difference among different dosage groups after radiotherapy(P>0.05). There was no significant difference between the expression of miR-150-5p before and after 2 Gy radiotherapy(P>0.05). While the relative level of miR-150-5p gradually decreased to 0.808, 0.605, 0.565 (χ2=18.76, P<0.05) with increased accumulative dosage of 10, 20, and 30 Gy, respectively. In addition, the miR-150-5p expression levels had no relationship(P>0.05)with situations (positive or negative group) of human epidermal growth factor receptor 2 (HER2), estrogen receptor (ER) and progesterone receptor (PR) in the breast cancer cells. Conclusions Ionizing radiation could reduce the expression of miR-150 in the plasma of breast cancer patients in a dose-dependent manner. |
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