| 胡凯,岳海英,刘文其,梁菲菲,王仁生.肿节风在小型猪放射性肺损伤过程中对TGF-β1/Smads表达水平的影响[J].中华放射医学与防护杂志,2016,36(3):178-185 |
| 肿节风在小型猪放射性肺损伤过程中对TGF-β1/Smads表达水平的影响 |
| Effect of Sarcandra Glabra on the expression of TGF-β1/Smads in miniature pig of radiation-induced lung injury |
| 投稿时间：2015-10-07 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.03.004 |
| 中文关键词: TGF-β1/Smads信号通路 放射性肺损伤 肿节风 小型猪 |
| 英文关键词:TGF-β1/Smads signaling pathway Radiation-induced lung injury Sarcandra Glabra Miniature pig |
| 基金项目:广西壮族自治区科学技术厅攻关项目(1140003A-24) |
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| 中文摘要: |
| 目的 研究肿节风在小型猪放射性肺损伤过程中对TGF-β1/Smads信号通路上各信号转导分子表达水平的影响。方法 75头小型猪按随机数字表法均分为健康对照组(不照射,给予生理盐水)、单纯照射组(右肺单次15 Gy γ射线+生理盐水)及药物+照射组(右肺单次15 Gy γ射线+肿节风)。分别于照射后2、4、8、12及24周取出右肺。应用HE染色观察肺组织病理改变,Real-time PCR及Western blot分别检测上述各时间点小型猪肺组织内TGF-β1、Smad2、Smad3和Smad7 mRNA及蛋白表达情况。结果 肿节风能显著减轻小型猪放射性肺损伤的炎症表现及纤维化程度。与健康对照组比较,单纯照射组TGF-β1和Smad3的表达水平从照射后第2周起、Smad2和Smad7的表达水平分别从照射后第8周和第12周起显著升高(P<0.05)。与单纯照射组比较,药物+照射组TGF-β1和Smad2的表达水平分别从照射后第4周和第8周起明显下降(P<0.05),Smad3的表达水平无明显变化,而Smad7的表达水平则从照射后第2周起显著升高(P<0.05)。结论 在放射性肺损伤的发病过程中,肿节风通过调节TGF-β1/Smads信号通路中主要信号转导分子TGF-β1、Smad2和Smad7的表达水平而发挥放射防护作用。 |
| 英文摘要: |
| Objective To study the effect of Sarcandra Glabra on the expression of signal transduction molecules of TGF-β1/Smads signaling pathway in miniature pig of radiation-induced lung injury. Methods 75 miniature pigs were divided into control group, radiation group and radiation plus medication group randomly. At 1 week before exposure of right lung with 15 Gy γ-rays, the miniature pigs in radiation plus medication group were given Sarcandra glabra, while those in the other groups received an equal amount of saline. Right lung were taken at weeks 2, 4, 8, 12 and 24 after irradiation, the pathological changes in the lung tissue were observed by HE staining, and the expression of mRNA and protein of TGF-β1, Smad2, Smad3, and Smad7 were detected by real-time PCR and western blotting, respectively. Results Sarcandra glabra reduced the inflammation and fibrosis of the lung tissue in miniature pig after irradiation. Compared with control group, the expressions of TGF-β1 and Smad3 were significantly increased at 2 weeks after irradiation(P<0.05), Smad2 and Smad7 were increased at 8 and 12 weeks after irradiation(P<0.05), respectively, in the radiation group. Compared with the radiation group, the expressions of TGF-β1 and Smad2 were significantly decreased(P<0.05) from the fourth and eighth week, respectively, Smad3 had no obvious change while Smad7 was significantly increased from the second week in the radiation plus medication group(P<0.05). Conclusions Sarcandra Glabra plays protective effect on radiation-induced lung injury in miniature pig by regulating TGF-β1, Smad2 and Smad7 expressions in the TGF-β1/Smads signaling pathway. |
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