| 陈军祥,朱国英,王建平,翟江龙.γ射线对人外周血单核细胞向破骨样细胞诱导分化的影响[J].中华放射医学与防护杂志,2016,36(3):173-177 |
| γ射线对人外周血单核细胞向破骨样细胞诱导分化的影响 |
| The effect of 137Cs γ-rays on the differentiation of human peripheral blood mononuclear cells to osteoclast-like cells |
| 投稿时间：2015-10-08 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.03.003 |
| 中文关键词: 电离辐射 人外周血单核细胞 破骨细胞分化 |
| 英文关键词:Ionizing radiation Human peripheral blood mononuclear cells Osteoclastogenesis |
| 基金项目:上海市自然科学基金(14ZR1401600);上海市卫生系统重要疾病联合攻关项目(2013ZYJB0801) |
|
| 摘要点击次数: 2636 |
| 全文下载次数: 1959 |
| 中文摘要: |
| 目的 探讨电离辐射对人外周血单核细胞来源的破骨细胞体外诱导分化的影响。方法 将人外周血采用密度梯度离心法获得单核细胞,经核因子κB受体活化因子配体(RANKL)和巨噬细胞集落刺激因子(M-CSF)诱导培养24 h,分别给予0、0.75和2 Gy的137Cs γ射线照射,第7天行TRAP染色后计数TRAP阳性多核破骨细胞,第10天取象牙骨片基质行甲苯胺蓝染色观察骨吸收陷窝形成;qRT-PCR检测破骨细胞特征标志物组织蛋白酶K和integrin β3的mRNA表达水平;ELISA法检测培养上清液中TRAcP-5b含量。结果 0.75 Gy照射组TRAP染色阳性多核破骨细胞计数显著多于对照组(0 Gy)(t=3.451,P<0.05),组织蛋白酶K和integrin β3的mRNA表达水平明显上调(t=2.343、2.728,P<0.05),且培养液中TRAcP-5b浓度明显增加(t=3.631,P<0.05)。而2 Gy照射组的TRAP染色阳性多核破骨细胞计数少于对照组,并伴有组织蛋白酶K和integrin β3的mRNA表达水平下调、培养上清液中TRAcP-5b浓度下降,但差异无统计学意义。结论 电离辐射可改变人外周血单核细胞破骨样细胞分化趋势,小剂量照射时可促进破骨细胞分化和骨吸收活性,而较大剂量照射后破骨细胞分化和骨吸收活性均降低。 |
| 英文摘要: |
| Objective To study the effect of γ-rays irradiation on the differentiation potential of the human peripheral blood monocytes (PBMCs) into osteoclast-like cells (OCLs) in vitro. MethodsPBMCs were isolated by density gradient centrifugation, treated by receptor activator of nuclear factor-κ B ligand (RANKL) and macrophage-colony stimulating factor (M-CSF) and exposed to 137Cs γ-rays with different radiation doses (0, 0.75, 2 Gy). After seven days of incubation, the cells were stained for tartrate resistant acid phosphatase (TRAP) and bone slices were stained by toluidine blue on the tenth day. Meanwhile, the characteristic osteoclast markers including Cathepsin K and integrin β3 were analyzed by real-time PCR. Tartrate resistant acid phosphatase 5b (TRAcP-5b) in the culture supernatant was determined by ELISA. Results PBMCs were differentiated into OCLs by the treatments of RANKL and M-CSF. The number of TRAP positive multinucleated OCLs was significantly higher in the dose of 0.75 Gy group than in control (0 Gy) group (t=3.451, P<0.05). Compared with the control group, the expression levels of Cathepsin K and integrin β3 and the concentration of TRAcP-5b were significantly elevated (t=2.343, 2.728, 3.631, P<0.05). However, in the 2 Gy group, there was a decrease in the number of osteoclasts, mRNA expression level of osteoclast characteristic markers and TRAcP-5b, but no statistically significant differences compared with the control group. Conclusions Ionizing radiation may influence the osteoclastogenesis during the PBMCs differentiation to OCLs. At low dosage, ionizing radiation promotes osteoclastogenesis and enhances the resorptive activity of osteoclasts, but a decline of differentiation potential was observed at high dosage of radiation. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|