| 许超,涂彧,周菊英,等.大鼠放射性脑损伤模型中Ca、Fe、Cu、Zn、Mg含量的变化及MgSO4的保护作用[J].中华放射医学与防护杂志,2016,36(2):87-93.Xu Chao,Tu Yu,Zhou Juying,et al.Dynamic changes of Ca, Fe, Cu, Zn, Mg in radiation-injured rat brain and radioprotective effect of MgSO4[J].Chin J Radiol Med Prot,2016,36(2):87-93 |
| 大鼠放射性脑损伤模型中Ca、Fe、Cu、Zn、Mg含量的变化及MgSO4的保护作用 |
| Dynamic changes of Ca, Fe, Cu, Zn, Mg in radiation-injured rat brain and radioprotective effect of MgSO4 |
| 投稿时间：2015-08-04 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.02.002 |
| 中文关键词: 放射性脑损伤 元素 硫酸镁 |
| 英文关键词:Radiation-induced brain injury Elements Magnesium |
| 基金项目:国家自然科学基金(81372921) |
|
| 摘要点击次数: 3506 |
| 全文下载次数: 2798 |
| 中文摘要: |
| 目的 观察全脑照射后大鼠脑组织含水量、微血管损伤及海马组织中Ca、Mg、Cu、Fe、Zn含量的动态变化,探讨这些元素在放射性脑损伤(RBI)发病机制中的作用。方法 用随机数字表法将大鼠分为空白组、防护组(400 mg/kg体重腹腔注射10%MgSO4溶液+单次全脑20 Gy电子束照射)和照射组(400 mg/kg体重腹腔注射生理盐水+单次全脑20 Gy电子束照射),每组18只,每个时间点3只。用干-湿重法测定脑组织含水量(BWC),HE染色观察脑组织微血管损伤程度,应用电感耦合等离子体发射光谱仪,定量分析大鼠脑放射性损伤后不同时间海马组织中Ca、Mg、Cu、Fe、Zn元素含量的动态变化。结果 照射组在照后7、14、30 d与空白组相比,脑含水量升高(t=-3.21、-2.11、2.82,P<0.05),防护组在照后7、14、30 d低于照射组(t=2.84、4.33、1.90,P<0.05)。RBI模型中出现微血管栓塞,防护组较照射组减轻。照射组Ca含量在照后各时间点及Fe含量在照后1、3、7 d与空白组相比含量升高(t=11.41、6.81、14.03、17.17、6.89、9.12和5.43、5.66、3.60,P<0.05),且照射组Ca含量在照后各时间点较防护组增加(t=5.35、5.28、11.02、14.26、5.68、9.10,P<0.05);照射组Cu含量在照后1、7、14、60 d及Zn含量在照后1、7、14、30、60 d低于空白组(t=4.24、3.76、4.76、3.86和5.25、4.78、26.53、6.67、11.37,P<0.05),防护组Cu含量在照后各时间点低于照射组(t=4.23、3.57、4.01、4.73、3.78、3.44,P<0.05),Zn含量在照后14 d高于照射组(t=6.21,P<0.05);照射组Mg含量在照后7 d降低(t=5.85,P<0.05)。结论 RBI模型中Ca、Fe、Cu、Zn含量失衡,补充MgSO4可以纠正Ca、Fe、Cu、Zn含量的失衡,并可以减轻放射性脑损伤。 |
| 英文摘要: |
| Objective To investigate the dynamic changes of the contents of brain water and Ca, Fe,Cu, Zn, Mg and microvascular damage in hippocampal tissue of radiation-injured rat brain. MethodsThe rats were randomly divided into control group, protective group (with intraperitoneal injection of 10% MgSO4, 400 mg/kg body weight+a single dose of 20 Gy electron beam irradiation in whole brain) and irradiation alone group (with intraperitoneal injection of normal saline, 400 mg/kg body weight+a single dose of 20 Gy electron beam irradiation to the entire brain) with 18 rats assigned to each group and 3 rats sampled at each time point. Radiation-induced brain injury (RBI) was modeled by irradiating the rat's whole brain with 5 MeV electrons. A dry-wet weight method was used to detect brain water content (BWC), and the level of microvascular damage was detected with HE staining of brain tissue slices, and the contents of Ca, Fe, Cu, Zn, Mg in hippocampus were detected with inductively coupled plasma atomic emission spectroscopy at different time points after radiation. Results BWC in the irradiated rats at 7, 14 and 30 d post-irradiation was higher than that of control group (t=3.21,-2.11,2.82, P<0.05), and BWC in the protective group was less than that in the irradiated group (t=2.84,4.33,1.90, P<0.05). Microvascular thrombosis was induced in the radiated brain but this thrombosis was reduced by MgSO4. The contents of Ca and Fe in the brain tissue after 1,3,7 d of irradiation was higher than that of control group (t=11.41, 6.81, 14.03, 17.17, 6.89, 9.12 and 5.43, 5.66, 3.60, P<0.05), and the content of Ca in the protective group at various time post-irradiation was less than that in the irradiated group (t=5.35, 5.28, 11.02, 14.26, 5.68, 9.10, P<0.05). The content of Cu (1, 7, 14,60 d post-irradiation) and Zn (1, 7, 14, 30, 60 d post-irradiation) of the irradiated group was less than that of the control group (t=4.24, 3.76, 4.76, 3.86 and 5.25, 4.78, 26.53, 6.67, 11.37, P<0.05), and the content of Cu in the protective group at different time points post-irradiation was less than that of the irradiated alone group (t=4.23, 3.57, 4.01, 4.73, 3.78, 3.44, P<0.05), the content of Zn in the protective group (14 d post-irradiation) was higher than that of the irradiated group (t=6.21, P<0.05). The content of Mg in the irradiated group (7 d post-irradiation) was less than that of the control group (t=5.85, P<0.05). Conclusions The contents of Ca, Fe,Cu and Zn were imbalanced in the radiation-injured rat brain, and the supplement of MgSO4 could recover the balance of Ca, Fe,Cu and Zn content and alleviate radiation-induced brain injury. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|