| 马洪鸽,林温文,史盼影,等.聚乙二醇和核酸适配体AS1411修饰的金纳米粒子对人宫颈癌HeLa细胞放射敏感性的影响[J].中华放射医学与防护杂志,2015,35(11):809-814.Ma Hongge,Lin Wenwen,Shi Panying,et al.Effects of AuNPs@PEG-AS1411 nanoparticles on radiosensitization of HeLa cancer cells[J].Chin J Radiol Med Prot,2015,35(11):809-814 |
| 聚乙二醇和核酸适配体AS1411修饰的金纳米粒子对人宫颈癌HeLa细胞放射敏感性的影响 |
| Effects of AuNPs@PEG-AS1411 nanoparticles on radiosensitization of HeLa cancer cells |
| 投稿时间：2015-04-19 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.11.003 |
| 中文关键词: AS1411 纳米金粒子 辐射敏感性 |
| 英文关键词:AS1411 Au nanoparticles Radiosensitization |
| 基金项目:江苏省高校优势学科建设(PAPD) |
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| 摘要点击次数: 5142 |
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| 中文摘要: |
| 目的 研究聚乙二醇(PEG)和核酸适配体AS1411修饰的金纳米粒子(AuNPs)对人宫颈癌HeLa细胞辐射敏感性的影响。方法 用PEG和PEG-AS1411分别修饰经柠檬酸钠还原法制备的AuNPs,制备纳米粒子AuNPs@PEG和AuNPs@PEG-AS1411。分别用CCK-8法和克隆形成法检测纳米粒子的细胞毒性。用电感耦合等离子体质谱仪(ICP-MS)检测HeLa细胞对纳米粒子的吸收量。用克隆形成法检测纳米粒子联合X射线照射对HeLa细胞存活率的影响。结果 CCK-8实验结果显示,AuNPs@PEG和AuNPs@PEG-AS1411对HeLa细胞的毒性很小(P>0.05),而克隆形成实验结果则显示,10 d后HeLa细胞的存活率明显降低(t=4.38~11.60,P<0.05)。用AS1411修饰AuNPs,可以增加细胞对AuNPs的吸收。AuNPs@PEG和AuNPs@PEG-AS1411对HeLa细胞均具有辐射增敏作用(F=7.90、48.23,P<0.05),Au浓度为10 mg/ L时,其增敏比分别为1.12和1.20。结论 AuNPs@PEG和AuNPs@PEG-AS1411对HeLa细胞的急性细胞毒性较小,但具有长期毒性。用AS1411修饰PEG化的AuNPs,可以增强AuNPs的放射增敏作用。 |
| 英文摘要: |
| Objective To study the effects of AuNPs@PEG-AS1411 nanoparticles on radiosensitization of human uterine cervix cancer HeLa cells.Methods AuNPs were synthesized by citrate reduction method and then functioned with PEG and PEG-AS1411, respectively. CCK-8 assay and colon forming assay were used to detect the acute and chronic toxicity effects of AuNPs on HeLa cells, respectively. At the same time, clonogenic survival assay was applied to measure the cell survival rate of HeLa cells after exposure to AuNPs@PEG and AuNPs@PEG-AS1411 combined with X-ray radiation. The intracellular uptake of AuNPs@PEG and AuNPs@PEG-AS1411 in HeLa cells were detected by ICP-MS. Results The CCK-8 assay showed that AuNPs@PEG and AuNPs@PEG-AS1411 were not toxical on HeLa cells(P>0.05). But the clonogenic survival assay showed that AuNPs@PEG and AuNPs@PEG-AS1411 had toxicity on HeLa cells significantly after 10 d(t=4.38-11.60,P<0.05). AuNPs functioned with AS1411 could increase the cellular uptake of AuNPs. AuNPs@PEG and AuNPs@PEG-AS1411 both had significant radiosensitive effect on HeLa cells(F=7.90,48.23,P<0.05). The values of SERDo for AuNPs@PEG and AuNPs@PEG-AS1411 were 1.12 and 1.20, respectively, when the concentration of Au was 10 mg/L. Conclusions AuNPs@PEG and AuNPs@PEG-AS1411 could cause chronic toxicity on HeLa cells instead of acute effect. PEGylated AuNPs functioned with AS1411 could enhance the radiosensitivity of HeLa cells in vitro. |
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