| 谢开红,胡小毛,刘平平,郑力,朱锦灿,刘善淘,何冬梅,刘革修.miR-155在淋巴瘤Raji细胞辐射抵抗中作用的研究[J].中华放射医学与防护杂志,2015,35(11):801-804 |
| miR-155在淋巴瘤Raji细胞辐射抵抗中作用的研究 |
| Role of miR-155 in radioresistance of lymphoma Raji cells |
| 投稿时间：2015-05-23 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.11.001 |
| 中文关键词: miR-155 Burkitt淋巴瘤 辐射 |
| 英文关键词:miR-155 Burkitt lymphoma Radiation |
| 基金项目:国家自然基金(81270568) |
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| 中文摘要: |
| 目的 研究miR-155在淋巴瘤Raji细胞辐射抵抗中的作用及其机制。方法 实时定量聚合酶链反应检测Raji细胞miR-155的表达,CCK-8法检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot检测PTEN和p-AKT的表达。结果 miR-155 RNA和p-AKT蛋白在Raji细胞中表达较高,而pten基因mRNA和蛋白质表达水平极低,单纯4.0 Gy照射48 h后Raji细胞凋亡率较低;miR-155 siRNA不仅使miR-155 RNA和p-AKT表达水平明显下降,而且pten基因mRNA和蛋白质水平显著增高,同时细胞显示出增殖抑制作用,显著增加Raji细胞对该辐射剂量敏感性,siRNA+射线照射组细胞凋亡率为(36.78±1.35)%,高于单纯照射组(t=12.572,P<0.05)。结论 miR-155 表达对Raji细胞辐射敏感性具有重要影响,其机制与PTEN/PI3K/AKT信号途径激活有关。 |
| 英文摘要: |
| Objective To investigate the role of miR-155 in radio-resistance of lymphoma cell line Raji cells.Methods The expression of miR-155 was detected by quantitative real-time PCR, cell growth was detected by CCK-8 assay, cell apoptosis was detected by flow cytometry, and the protein expressions of PTEN and p-AKT were detected by Western blot. Results The Raji cells had high levels of miR-155 RNA and p-AKT protein had a very low level of PTEN protein. Cell apoptosis was induced by 4.0 Gy X-ray irradiation after 48 h. The transfection of cells with miR-155 siRNA not only silenced miR-155, but also significantly decreased p-AKT level, increased the expression of pten gene and protein level, inhibited cell proliferation, and increased the radiation sensitivity of Raji cell, the apoptosis rate of siRNA+ irradiation group(36.78%±1.35%) was higher than that of irradiation group(t=12.572,P<0.05).Conclusions High levels of miR-155 was involved in radiation resistance of lymphoma Raji cells and this activity is likely modulated by the PTEN/PI3K/AKT signaling pathway. |
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