| 刘冬梅,吴慧,马艳会,张文广.双向调控Cox-2表达对人食管癌EC9706细胞裸鼠移植瘤放射敏感性的影响[J].中华放射医学与防护杂志,2015,35(10):734-737 |
| 双向调控Cox-2表达对人食管癌EC9706细胞裸鼠移植瘤放射敏感性的影响 |
| Effects of up and down-regulation of Cox-2 expression on radiation sensitivity of human esophageal cancer EC9706 xenograft in nude mice |
| 投稿时间：2015-06-17 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.10.004 |
| 中文关键词: 食管癌 EC9706细胞 Cox-2 移植瘤 裸鼠 |
| 英文关键词:Esophageal cancer EC9706 Cox-2 Xenograft tumor Nude mice |
| 基金项目:河南省医学科技攻关计划项目(200903144) |
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| 中文摘要: |
| 目的 探讨上调和下调Cox-2基因表达对食管癌EC9706细胞裸鼠移植瘤放射敏感性的影响。方法 构建针对Cox-2基因的siRNA载体及Cox-2基因真核表达载体,通过脂质体转染技术将其转入食管癌EC9706细胞,G418筛选得到稳定转染细胞系。荧光定量RT-PCR和Western blot分别检测细胞中Cox-2 mRNA和Cox-2蛋白表达水平;裸鼠移植瘤实验检测上调和下调Cox-2表达联合X射线照射对食管癌的生长抑制作用。结果 Cox-2下调组食管癌EC9706细胞内Cox-2基因表达明显降低,Cox-2上调组明显升高。Cox-2下调组裸鼠移植瘤平均体积明显小于对照组(F=34.26,P<0.05);Cox-2上调组的瘤体平均体积大于对照组(F=26.38,P<0.05)。20 Gy γ射线照射后Cox-2下调组瘤体平均体积较照射前明显减小(F=16.35,P<0.05);而上调组裸鼠皮下种植瘤平均体积较照射前无明显变化。结论 下调Cox-2表达能抑制人食管癌EC9706细胞裸鼠移植瘤的生长,增强瘤体的放射敏感性,上调Cox-2表达则使肿瘤辐射抗拒。 |
| 英文摘要: |
| Objective To explore the effects of up- and down-regulation of Cox-2 expression on the growth and radiation sensitivity of human esophageal cancer EC9706 xenograft in nude mice. Methods Cox-2 specific siRNA and Cox-2 gene eukaryotic expression vector were constructed and transfected to esophageal cancer cells EC9706, and the stable transfected cell lines were obtained by the method of G418 screening. The expressions of Cox-2 mRNA and its protein were detected by RT-PCR and Western blot, respectively. The inhibitory effects of Cox-2 regulation combined with X-ray irradiation on cancer cell growth were detected by the nude mouse xenograft assay. Results Cox-2 gene expression was significantly decreased in the Cox-2 down-regulated group and increased in the Cox-2 up-regulated group. Compared with the control group without gene transfer, the average volumes of EC9706 xenograft tumor in the Cox-2 up- and down-regulated group significantly decreased (F=34.26,P<0.05) and increased (F=26.38,P<0.05), respectively. After 20 Gy X-ray irradiation, the average volume of xenograft was significantly reduced in the Cox-2 down-regulated group (F=16.35,P<0.05), but it had no significantly changes in the Cox-2 up-regulated group. Conclusions Down-regulation of Cox-2 expression inhibits the growth of human esophageal cancer EC9706 xenograft in a nude mice and increases cell radiation sensitivity, but up-regulation of Cox-2 expression makes tumor cells to become radioresistant. |
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