| 邵帅,张娴勰,马增春,等.四物汤有效成分配伍组方对电离辐射所致氧化损伤的防护作用[J].中华放射医学与防护杂志,2015,35(10):725-729.Shao Shuai,Zhang Xianxie,Ma Zengchun,et al.The protective effect of Siwu Tang formulation on oxidative damage caused by ionizing radiation[J].Chin J Radiol Med Prot,2015,35(10):725-729 |
| 四物汤有效成分配伍组方对电离辐射所致氧化损伤的防护作用 |
| The protective effect of Siwu Tang formulation on oxidative damage caused by ionizing radiation |
| 投稿时间：2015-03-13 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.10.002 |
| 中文关键词: 四物汤有效成分配伍组方 电离辐射 AHH-1细胞 抗氧化 Nrf2-Keap1-ARE信号通路 |
| 英文关键词:Siwu Tang formulation Ionizing radiation AHH-1 cells Antioxidant Nrf2-Keap1-ARE signaling pathway |
| 基金项目:国家自然科学基金(81130067,81303316) |
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| 中文摘要: |
| 目的 观察四物汤有效成分配伍组方对电离辐射所致人淋巴母细胞AHH-1细胞氧化损伤的辐射防护作用,并初步探讨其作用机制。方法 4.0 Gy 60Co γ射线单次照射AHH-1细胞,造成电离辐射损伤。观察不同浓度的四物汤有效成分配伍组方(果糖、阿魏酸、芍药苷、川芎嗪质量比为9 008:53:721:267)对于电离辐射损伤的AHH-1细胞活力、凋亡情况、活性氧(ROS)水平的影响,以及对pGL4-ARE报告基因载体的激活作用。结果 四物汤有效成分配伍组方在10 mg/L的浓度下抗辐射效果最为显著,可以减缓受照AHH-1细胞活力下降(t=-8.152,P<0.05),缓解早期细胞凋亡(t=12.777,P<0.05),降低ROS水平(t=3.465,P<0.05),诱导报告基因pGL4-ARE荧光表达倍数提高(t=-19.752,P<0.05)。结论 四物汤有效成分配伍组方具有体外抗辐射活性,可能通过激活Nrf2-Keap1-ARE抗氧化通路、降低辐射所致氧化损伤,从而发挥辐射防护作用。 |
| 英文摘要: |
| Objective To observe the effects of Siwu Tang formulation, which consists of fructose, ferulic acid, paeoniflorin, and ligustrazine hydrochloride (mass ratio of 9 008:53:721:267), on the oxidative damage in irradiated AHH-1 cells, and to investigate the underlying protective mechanism. Methods AHH-1 cells were irradiated with a single dose of 4.0 Gy 60Co γ-rays, then cell viability, apoptosis and reactive oxygen species(ROS) level were measured. The activation of Nrf2-Keapl-ARE pathway was observed by transferring pGL4-ARE reporter gene transiently into HEK293 cells before the administration of Siwu Tang. Results At the concentration of 10 mg/L, Siwu Tang components formula had the best efficiency on anti-radiation damage. This drug inhibited the reduction of cell viability (t=-8.152, P<0.05), mitigated apoptosis (t=12.777, P<0.05), reduced intracellular ROS levels (t=3.465, P<0.05), and induced the expression of pGL4-ARE fluorescence reporter gene (t=-19.752, P<0.05). Conclusions Siwu Tang formulation has a protective effect against radiation by reducing radiation-induced oxidative damage through the activation of the Nrf2-Keap1-ARE signaling pathway. |
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