| 张朦,熊耀祖,涂彧,周菊英.大鼠放射性脑损伤模型中核转录因子-κB表达的动态变化规律[J].中华放射医学与防护杂志,2015,35(9):657-661 |
| 大鼠放射性脑损伤模型中核转录因子-κB表达的动态变化规律 |
| The dynamic changes of nuclear factor-κB during brain injury of irradiated rats |
| 投稿时间：2015-03-05 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.09.004 |
| 中文关键词: 核转录因子-κB 放射性脑损伤 动物模型 |
| 英文关键词:Nuclear factor-κB Radiation-induced brain injury Animal model |
| 基金项目:国家自然科学基金(81372929) |
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| 中文摘要: |
| 目的 探讨放射性脑损伤发生过程中核转录因子-κB(NF-κB)的表达变化。方法 健康SD大鼠82只,按随机数字表法分为健康对照组(32只,给予相同的麻醉但不照射)和照射组(50只,单次全脑照射20 Gy建立放射性脑损伤模型),分别于照射后1、3、7、14、28 d处死,断头取脑。通过实时荧光定量反转录聚合酶链反应(QRT-PCR)检测NF-κB mRNA表达变化,用ELISA及Western blot检测NF-κB蛋白质表达情况,免疫组织化学染色观察NF-κB蛋白在海马区域的定位表达情况。结果 照射组NF-κB mRNA水平在照后开始升高,第3天达到表达高峰,其中第3、7天表达量与健康对照组相比,差异有统计学意义(t=37.79、35.30,P<0.05)。NF-κB蛋白的表达在照后第1天即达最高峰,随后开始逐渐下降,至照后第28天恢复正常水平。ELISA结果显示,与健康对照组相比,受照后第1、3、7、14天蛋白表达差异有统计学意义(t=30.94、14.87、27.17、13.27,P<0.05)。免疫组织化学染色结果显示,在受照后第1、3、7天海马区域的NF-κB染色强度增强,阳性表达细胞数量增多,与健康对照组相比,差异有统计学意义(t=-8.49、-4.47、-3.46,P<0.05)。结论 NF-κB的基因及蛋白水平表达趋势均先升高后降低,促进了放射性脑损伤的发生发展。 |
| 英文摘要: |
| Objective To investigate the dynamic changes of nuclear factor-κB (NF-κB) during brain injury of irradiated rats. Methods According to the random number table method, 82 healthy Sprague-Dawley rats were divided into two groups: 50 rats in the irradiation group and the other 32 rats in the control group given sham-irradiation. The whole brains of rats in the irradiation group were irradiated with a single dose of 20 Gy electron beam to establish an animal model of radiation-induced brain injury. All brain tissues were respectively taken out 1, 3, 7, 14 and 28 d after irradiation. The quantificational real-time-polymerase chain reaction (QRT-PCR) was used to detect the expressions of the NF-κB mRNA while the expression of NF-κB protein was analyzed by ELISA and Western blot. The positioning expression of NF-κB protein in the hippocampus was measured with immunohistochemical staining. Results In the irradiation group, the expression of NF-κB mRNA significantly increased at 3 and 7 d following irradiation (t=37.79, 35.30, P<0.05) and it approached a peak value on the third day following irradiation. The expression of NF-κB protein in whole brain tissues after irradiation was up-regulated and reached a peak on the first day post-irradiation and it was significantly higher than controls at 1, 3, 7, 14 d after irradiation (t=30.94, 14.87, 27.17, 13.27, P<0.05) then declined and dropped to the normal level by 28 d. The results of immunohistochemical staining showed that the number of NF-κB positive cells in the hippocampus increased at 1, 3 and 7 d following irradiation (t=-8.49, -4.47, -3.46, P<0.05). Conclusions After irradiation, the expressions of NF-κB mRNA and corresponding protein first increase and then later decrease, which promotes the development of brain injury. |
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