| 岳海英,胡凯,蒋健,陈玉华,刘文其,王仁生.小型猪右胸单次大剂量γ射线照射后的肺损伤研究[J].中华放射医学与防护杂志,2015,35(8):561-565 |
| 小型猪右胸单次大剂量γ射线照射后的肺损伤研究 |
| The study on lung injury of minipigs' right-thorax with a single high-dose γ-ray irradiation |
| 投稿时间：2015-01-06 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.08.001 |
| 中文关键词: 放疗 小型猪 肺泡Ⅱ型上皮细胞 上皮细胞向间质细胞转化 |
| 英文关键词:Radiotherapy Minipigs Alveolar epithelial type Ⅱ cells Epithelial-mesenchymal transition |
| 基金项目:人口健康与食品安全关键技术研究开发基金(桂科攻1140003A-24) |
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| 中文摘要: |
| 目的 探讨巴马小型猪经右胸15 Gy的单次照射后肺泡Ⅱ型上皮细胞中细胞因子及蛋白的变化和可能的作用。方法 将40只雄性巴马小型猪按随机数字表法分为健康对照组(不予照射)和照射组(60Co γ射线进行右胸15 Gy单次照射)。照射后4、8、12、24周,从两组中分别取5只采集右侧肺组织,行HE染色观察肺组织病理变化;免疫组织化学染色观察α-平滑肌肌动蛋白(α-SMA蛋白)表达;免疫印迹检测肺表面活性物质相关蛋白A(SP-A)、转化生长因子β1(TGF-β1)、波形蛋白(Vimentin)和E-钙黏蛋白(E-cadherin)的蛋白表达;免疫荧光双染观察α-SMA和SP-A的共表达。结果 照射后的4、8、12和24周,照射组中α-SMA、TGF-β1和Vimentin的蛋白表达较健康对照组明显升高(α-SMA:t=2.46~3.26,P<0.05;TGF-β1:t=2.96~3.52,P<0.05;Vimentin:t=3.24~5.05,P<0.05);而SP-A和E-cadherin的蛋白表达低于健康对照组(SP-A:t=3.62~4.65,P<0.05;E-cadherin:t=2.53~4.15,P<0.05)。照射后8周,照射组可见肺泡Ⅱ型上皮细胞的α-SMA和SP-A蛋白共染,而健康对照组中未见。结论 E-cadherin、TGF-β1和SP-A可以作为放射性肺损伤发生的预测指标,而肺泡Ⅱ型上皮细胞可通过向间质细胞转化参与放射性肺损伤的发生发展。 |
| 英文摘要: |
| Objective To explore the influence and mechanism of cytokines and protein expression of alveolar epithelial type (ACE) II cells in Bama minipigs' right-thorax with a single 15 Gy dose irradiation. Methods All minipigs received either right thoracic irradiation or sham-irradiation under anesthesia. At 4, 8, 12 and 24 week post-irradiation, 5 minipigs respective and random from irradiarion groups and control group were sacrificed to remove the lungs. The protein expression of surfactant associated protein (SP)-A, transforming growth factor (TGF)-β1, Vimentin and E-cadherin were detected by Western blot. The protein expression of α-smooth muscle actin (SMA) was detected by immunohistochemistry. The co-localization of SP-A and α-SMA was visualized by double immunofluorescence staining. Results At 4, 8, 12 and 24 week post-irradiation, a significant increase in the protein expression of α-SMA, TGF-β1 and Vimentin were observed in irradiated lung compared to sham-irradiated controls(α-SMA: t=2.46-3.26, P<0.05; TGF-β1: t=2.96-3.52, P<0.05; Vimentin: t=3.24-5.05, P<0.05). By contrast, the protein expression of SP-A and E-cadherin in irradiation group was lower than it in control group(SP-A: t=3.62-4.65,P<0.05;E-cadherin:t=2.53-4.15,P<0.05). Moreover, at 8 week after irradiation, under confocal laser scanning microscope, the co-localization of SP-A and α-SMA was observed in irradiated alveolar epithelium cells, and it was not observed in sham-irradiated controls. Conclusions These data demonstrate that E-cadherin, SP-A and TGF-β1 may act as sensitive predictors of radiation-induced lung injury(RILI). Irradiation may lead to ACEⅡ cells achieving a mesenchymal phenotype, namely, epithelial to mesenchymal cells transition occurs, and ACEⅡ cells play the important part in the development of RILI by epithelial-mesenchymal transition. |
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