| 王俊可,韩阿如娜,王丽梅,等.rhG-CSF大剂量单次给药对非清髓骨髓移植小鼠造血功能重建的影响[J].中华放射医学与防护杂志,2015,35(7):501-504.Wang Junke,Han Aruna,Wang Limei,et al.The influence of a single high dose of rhG-CSF on hematopoietic reconstitution of mice with nonmyeloablative bone marrow transplantation[J].Chin J Radiol Med Prot,2015,35(7):501-504 |
| rhG-CSF大剂量单次给药对非清髓骨髓移植小鼠造血功能重建的影响 |
| The influence of a single high dose of rhG-CSF on hematopoietic reconstitution of mice with nonmyeloablative bone marrow transplantation |
| 投稿时间：2015-02-05 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.07.005 |
| 中文关键词: 非清髓 骨髓移植 造血干细胞 重组人粒细胞集落刺激因子 |
| 英文关键词:Nonmyeloablative Bone marrow transplantation Hemopoietic stell cell rhG-CSF |
| 基金项目: |
|
| 摘要点击次数: 4407 |
| 全文下载次数: 3064 |
| 中文摘要: |
| 目的 观察重组人粒细胞集落刺激因子(rhG-CSF)大剂量单次给药对非清髓骨髓移植小鼠造血恢复的影响,为rhG-CSF的临床应用提供新的实验依据。 方法 雄性C57BL/6J小鼠,经60Co γ射线6 Gy全身照射。于受照后0.5 h给予rhG-CSF处理,通过检测接受不同骨髓移植时间(未移植组和照后0.5、6、24和48 h移植组)和rhG-CSF不同给药剂量(0、0.25、0.5和1 mg/kg组)照射小鼠外周血象变化,摸索最佳接受骨髓移植时间和rhG-CSF最佳给药剂量。建立绿色荧光蛋白(GFP)小鼠非清髓骨髓移植小鼠模型,通过流式细胞仪检测受体小鼠外周血中GFP+白细胞占有核细胞总数的百分数,探究rhG-CSF大剂量单次给药对外源性干祖细胞植入的影响。结果 rhG-CSF大剂量单次给药对照后0.5~48 h内接受非清髓骨髓移植小鼠的造血恢复均有不同程度的促进作用,其中对照后24 h移植组小鼠的白细胞、红细胞和血小板的恢复最佳(F=17.76、14.75、8.66, P<0.05);0.25~1.0 mg/kg rhG-CSF单次给药可不同程度地促进非清髓骨髓移植小鼠的多系造血恢复,尤以大剂量rhG-CSF(1.0 mg/kg)给药时效果更为显著(F=5.34、8.92、16.54, P<0.05);此外,照射小鼠接受GFP小鼠骨髓细胞移植后,在照后9~21 d,rhG-CSF组小鼠外周血白细胞数均明显高于移植对照组(F=35.61, P<0.05),但GFP+细胞比例在给药组与移植对照组之间差异无统计学意义。 结论 rhG-CSF大剂量单次给药可显著促进非清髓骨髓移植小鼠多系造血功能恢复,但对输入的外源造血干细胞植入率没有明显影响。 |
| 英文摘要: |
| Objective To observe the peripheral hematopoietic recovery after a high dose of rhG-CSF administration on the mice with nonmyeloablative bonw marrow transplantation (BMT), and provide new experimental evidence for the clinical application of rhG-CSF. Methods Male C57 mice were treated with rhG-CSF at 0.5 h after whole body γ-ray irradiation and then were randomly divided into 5 groups with different time of BMT after irradiation (vehicle, 0.5, 6, 24, 48 h) and 4 groups with different dosage of rhG-CSF (0, 0.25, 0.5, and 1 mg/kg). The amount of karyocyte was analyzed to evaluate the best BMT time and dosage of rhG-CSF. To explore the effect of rhG-CSF on the implantation of exogenous stem/progenitor cells, a model of BMT with GFP+ fluorescent mice was established, in which the percentage of GFP+ leucocyte cells of the total peripheral blood nucleated cells of recipient mice was tested by flow cytometry. Results The administration of rhG-CSF could accelerate multi-lineage hematopoietic recovery, including white blood cells, red blood cells and platelets on mice with nonmyeloablative BMT (F=17.76,14.75,8.66, P<0.05). The optizum time of BMT was 24 h after irradiation and the best dosage of rhG-CSF was 1 mg/kg (F=5.34,8.92,16.54,P<0.05). In addition, in the irradiated mice receiving BMT with GFP mouse cells, the amount of peripheral white blood cells in the rhG-CSF group was significantly higher than those of control mice in 9~21 d (F=35.61, P<0.05), while the proportion of GFP+ cells between these two groups had no significant difference. Conclusions A single high dose of rhG-CSF can significantly promote multi-lineage hematopoietic recovery on mice with nonmyeloablative BMT, while the implantation rate of exogenous hematopoietic stem cells has no obvious influence. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|