| 卢小迪,朱小东,赵伟,曲颂,梁霞,苏芳.自噬活性的降低增加人鼻咽癌CNE-2细胞的放射敏感性[J].中华放射医学与防护杂志,2015,35(6):423-427 |
| 自噬活性的降低增加人鼻咽癌CNE-2细胞的放射敏感性 |
| Suppression of autophagy activity enhances the radiosensitivity of human nasopharyngeal carcinoma CNE-2 cells |
| 投稿时间：2014-11-17 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.06.005 |
| 中文关键词: ATG5 自噬 鼻咽癌 放射敏感性 |
| 英文关键词:ATG5 Autophagy Nasopharyngeal carcinoma Radiosensitivity |
| 基金项目:国家自然科学基金(81160285);广西壮族自治区自然科学基金(桂科自2010gxnsfa013240) |
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| 中文摘要: |
| 目的 研究自噬与人鼻咽癌CNE-2细胞放射敏感性之间的关系。方法 采用慢病毒介导的RNA干扰技术建立稳定沉默自噬相关基因ATG5的人鼻咽癌CNE-2细胞系,实验分为未转染的CNE-2细胞组(对照组)、转染NC-shRNA的CNE-2细胞组(NC组)及转染ATG5-shRNA的CNE-2细胞组(ATG5组),应用CCK-8法、流式细胞术及克隆形成实验检测细胞增殖、凋亡及放射敏感性的变化。结果 CCK-8实验结果显示,与对照组和NC组相比,各剂量点ATG5组的细胞存活率均显著降低(F=3.755、46.086、8.609、44.160,P<0.05),绘制细胞生存曲线可见下调ATG5的表达后可以增加CNE-2细胞的放射敏感性;流式细胞术结果显示,经6 Gy X射线照射后,ATG5组细胞的凋亡率较NC组及对照组明显升高(F=394.876,P<0.05);克隆形成实验结果提示沉默ATG5基因可增加鼻咽癌CNE-2细胞的放射敏感性。结论 降低鼻咽癌CNE-2细胞的自噬活性可以增强其放射敏感性。 |
| 英文摘要: |
| Objective To investigate the effect of autophagy in radiosensitivity of nasopharyngeal carcinoma CNE-2 cells. Methods The expression of ATG5 in CNE-2 cells was inhibited by lentiviral mediated RNA interference. The cells were divided into three groups: control group, NC group and ATG5 group. Cell proliferation, apoptosis and radiosensitivity were detected by CCK-8 method, flow cytometry and colony-forming assay, respectively. Results Compared with the NC group and control group, the survival of ATG5-interfected cells was reduced (F=3.755,46.086,8.609,44.160,P<0.05). After 6 Gy X-ray irradiation, the apoptosis index of the ATG5 group significantly higher than that of NC group and control group (F=394.876,P<0.05). In addition, the radiosensitivity of ATG5 group was also higher than that of control cells. Conclusions Suppression of autophagy activity enhances the radiosensitivity of human nasopharyngeal carcinoma cells. |
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