| 郭亚,朱小东,曲颂,李龄,葛莲英,黄诗婷,赵伟,苏芳,李烨.沉默STAT1基因增强放射抗拒鼻咽癌CNE-2R细胞放射敏感性[J].中华放射医学与防护杂志,2015,35(5):334-338 |
| 沉默STAT1基因增强放射抗拒鼻咽癌CNE-2R细胞放射敏感性 |
| Silencing STAT1 gene enhances radiosensitivity of radioresistant human nasopharyngeal carcinoma CNE-2R cells |
| 投稿时间：2014-11-08 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.05.004 |
| 中文关键词: STAT1 RNA干扰 慢病毒载体 放射敏感性 鼻咽癌 |
| 英文关键词:STAT1 RNA interference Lentiviral vector Radiosensitivity Nasopharyngeal carcinoma |
| 基金项目:国家自然科学基金(30860329);广西壮族自治区自然科学基金(桂科自0832229);广西重点实验室专项经费资助项目(GK2014-ZZ09);广西高等学校重大科研资助项目(201101ZD004) |
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| 中文摘要: |
| 目的 研究基因STAT1沉默对人放射抗拒鼻咽癌细胞放射敏感性的影响.方法 慢病毒介导的STAT1基因转染放射抗拒鼻咽癌细胞CNE-2R,荧光定量RT-PCR技术检测沉默效果.MTT法检测转染前后细胞增殖活性,流式细胞技术检测细胞周期及凋亡,克隆形成实验检测转染前后细胞放射敏感性变化.结果 慢病毒转染后放射抗拒鼻咽癌细胞STAT1表达降低(F=429.87,P<0.05)、细胞生长抑制(F3=.88~4.63,P<0.05)、凋亡率增加(F=38.13,P<0.05)、放射敏感性增加(F=252.80,P<0.05)、细胞周期中G0/G1、S和G2/M期未见明显差异(P>0.05).结论 沉默STAT1基因能增加放射抗拒鼻咽癌细胞 CNE-2R的放射敏感性. |
| 英文摘要: |
| Objective To study the effect of silencing STAT1 on radiation sensitivity of nasopharyngeal carcinoma cells. Methods Lentivirus-mediate STAT1 gene-transfer was performed to radioresistant human nasopharyngeal carcinoma CNE-2R cells and the transfection efficiency was detected by RT-PCR. The cell proliferation was measured by MTT, and the apoptosis was analyzed by flow cytometry. The radiosensitization ratio was measured according to the clone formation efficiency. Results After transfection with STAT1 siRNA, the expression of STAT1 was effectively inhibited in CNE-2R cells(F=429.87, P<0.05), the cell proliferation was decreased (F=3.88-4.63, P<0.05), the apoptosis was increased (F=38.13,P<0.05), the cell radiosensitivity was enhanced (F=252.80,P<0.05), but the cell cycle distribution at G0/G1, S and G2/M were not influenced (P>0.05).Conclusions Silencing STAT1 could increase radiosensitization of nasopharyngeal carcinoma cells. |
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