| 靳瑾,白佳利,龙伟,沈秀,徐文清,周则卫.吡咯烷二硫代氨基甲酸盐对急性辐射损伤小鼠的防护作用[J].中华放射医学与防护杂志,2015,35(4):257-260 |
| 吡咯烷二硫代氨基甲酸盐对急性辐射损伤小鼠的防护作用 |
| The protective effect of pyrrolidine dithiocarbamate on acute radiation injury in mice |
| 投稿时间：2014-09-05 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.04.005 |
| 中文关键词: 吡咯烷二硫代氨基甲酸盐 γ射线 ICR小鼠 辐射损伤 |
| 英文关键词:Pyrrolidine dithiocarbamate γ-rays ICR mice Radiation injury |
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| 目的 研究吡咯烷二硫代氨基甲酸盐(pyrrolidine dithiocarbamate,PDTC)对急性辐射损伤小鼠的防护作用.方法 6~8周龄雄性ICR小鼠,按体重随机分为5组,每组10只:空白单照组(IR)、阳性对照组(氨磷汀WR-2721 250 mg/kg)和PDTC30、60、90 mg/kg剂量组,照射前30 min给予相应药品.137Cs γ射线7.5 Gy一次性全身照射,观察小鼠30 d存活率;137Cs γ射线5.0 Gy一次性全身照射,照射后第8天检测外周血、造血系统、脏器系数指标.结果 PDTC可以提高小鼠30 d存活率,60 mg/kg剂量组效果最为明显,存活率提高到60%(6/10),空白单照组为10%(1/10),阳性对照组为70%(7/10).与空白单照组相比,PDTC 60 mg/kg剂量组的脾脏指数、白细胞、血红蛋白含量、血小板数、股骨有核数和脾结节数,差异有统计学意义(t=2.354、4.793、2.342、6.542、2.649、3.982,P<0.05).结论 PDTC具有抗辐射损伤作用,尤以60 mg/kg作用最为明显. |
| 英文摘要: |
| Objective To study the protective effect of pyrrolidine dithiocarbamate (PDTC) on acute irradiated mice. Methods The 6-8 weeks old male ICR mice were randomly divided into five groups: irradiation alone group (IR), positive control group (amifostine WR-2721 250 mg/kg) and PDTC of 30, 60 and 90 mg/kg dose groups. Each group had 10 mice and the drug was given at 0.5 h before whole body irradiation. At 30 d post-irradiation of 7.5 Gy 137Cs γ-rays, the mice survival were observed. At 8 d post-irradiation of 5.0 Gy 137Cs γ-rays, the peripheral blood, hematopoietic system and organ indexes were observed to evaluate the radiation protective effect of PDTC. Results PDTC increased the 30-day survival rates and 60 mg/kg dose had the most obvious effect by increase the survival to 60%(6/10). The survivals of irradiation alone group and the amifostine positive control group was 10%(1/10) and 70%(7/10), respectively. Compared with the irradiation alone group, 60 mg/kg PDTC group had the significant difference in spleen index, WBC, HGB, PLT, bone marrow nucleated cells and colony forming unit of spleen(t=2.354, 4.793, 2.342, 6.542, 2.649, 3.982, P<0.05). Conclusions PDTC is effective in radiation protection with an optimum dose of 60 mg/kg. |
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