| 高辉,左斯尧,黄政基,等.不同剂量辐射诱导小鼠胸腺Th1-Th2-Th3型细胞相关基因的功能分析[J].中华放射医学与防护杂志,2015,35(4):248-251.Gao Hui,Zuo Siyao,Huang Zhengji,et al.Analysis of Th1-Th2-Th3 related gene expressions in the thymus of mice irradiated with different doses[J].Chin J Radiol Med Prot,2015,35(4):248-251 |
| 不同剂量辐射诱导小鼠胸腺Th1-Th2-Th3型细胞相关基因的功能分析 |
| Analysis of Th1-Th2-Th3 related gene expressions in the thymus of mice irradiated with different doses |
| 投稿时间：2014-08-29 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.04.003 |
| 中文关键词: 辐射 剂量 辅助性T细胞 功能分类芯片 免疫应答 |
| 英文关键词:Radiation Dose Th cells PCR array Immune response |
| 基金项目:国家自然科学基金(30870584,81371890);教育部高等学校博士学科点专项科研基金(20120061110063) |
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| 中文摘要: |
| 目的 应用功能分类基因芯片技术,分析高、低剂量全身照射对小鼠胸腺细胞中Th1、Th2及Th3/Tr1各亚型功能相关基因的差异表达,探讨辐射免疫效应的分子机制.方法 健康ICR小鼠按随机数字表法分为低剂量组(0.075 Gy)、高剂量组(2.0 Gy)和假照组,于照射后16 h处死小鼠取胸腺组织,应用细胞因子与炎症反应PCR芯片技术进行Th1-Th2-Th3功能分类芯片分析.结果 低剂量(0.075 Gy)X射线全身照射后小鼠胸腺细胞中有8个基因表达上调,5个基因表达下调;高剂量(2.0 Gy)X射线全身照射后小鼠胸腺细胞中有54个基因表达上调,3个基因表达下调.具体有Th型细胞相关基因、Th2型细胞相关基因、Th3/Tr1型细胞相关基因、Th1/Th2型免疫应答基因以及转录因子相关基因.其中,低剂量辐射诱导胸腺中的Th1型细胞相关基因Stat4和Socs1的表达上调,而对Th2型和Th3/Tr型细胞相关基因IL-4ra、Cebpb、Gata3及Tgfb3下调,最终导致Th1型免疫应答基因Sftpd上调.高剂量辐射均可诱导Th1、Th2和Th3/Tr型细胞相关基因的上调,但Th1型免疫应答基因表达无变化,而Th2型免疫应答相关基因Cd86、IL-18、IL-10以及Irf4上调.结论 低剂量辐射诱导Th1型免疫应答,而高剂量辐射诱导Th2型免疫应答. |
| 英文摘要: |
| Objective To analyze the effect of high and low dose radiation on the expressions of Th1, Th2 and Th3 /Tr1 related-genes in mice thymocytes and investigate the possible underlying molecular mechanism. Methods ICR mice were randomly divided into low-dose group (0.075 Gy), high-dose group (2.0 Gy) and sham-control group. The mouse thymus tissue was extracted at 16 hours after irradiation and the expressions of Th1-Th2-Th3 related genes were measured by PCR array. Results Eight genes were up-regulated and five genes were down-regulated after low dose radiation (0.075 Gy); while 54 genes were up-regulated and three genes were down-regulated after high dose (2.0 Gy) radiation. These genes included Th1 cell related genes, Th2 cell related genes, Th3/Tr1 cell related genes, Th1/Th2 immune response genes and transcription factor related genes. Low dose radiation induced up-regulation of Stat4 and Socs1 of genes related to the Th1 cells, and it induced down-regulation of IL-4ra, Cebpb, Gata3 and Tgfb3 associated with Th2 and Th3 cells, which lead to Sftpd genes up-regulation of Th1 immune response eventually. The high dose radiation up-regulated all of Th1, Th2 and Th3/Tr related genes and also enhanced the expressions of Cd86, IL-18, IL-10 and Irf4 genes related to Th2 immune response, but it did not alter the gene expression of Th1 immune response. Conclusions Low-dose radiation induces Th1-type immune response, while high doses radiation triggers Th2 type immune response. |
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