| 冯建刚,李爽,封江彬,等.人淋巴细胞S100A4基因在照射后不同时间点表达水平的剂量-效应关系[J].中华放射医学与防护杂志,2015,35(4):245-247,302.Feng Jiangang,Li Shuang,Feng Jiangbin,et al.Dose-response of S100A4 gene expression in human lymphocytes at different time points after irradiation[J].Chin J Radiol Med Prot,2015,35(4):245-247,302 |
| 人淋巴细胞S100A4基因在照射后不同时间点表达水平的剂量-效应关系 |
| Dose-response of S100A4 gene expression in human lymphocytes at different time points after irradiation |
| 投稿时间：2014-10-03 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.04.002 |
| 中文关键词: 实时荧光PCR S100A4 基因表达 60Co γ射线 |
| 英文关键词:Real-time PCR S100A4 Gene expression 60Co γ-rays |
| 基金项目:国家自然科学基金(81172593) |
|
| 摘要点击次数: 3395 |
| 全文下载次数: 2803 |
| 中文摘要: |
| 目的 应用实时荧光PCR技术检测不同剂量60Co γ射线照射后不同时间永生化淋巴细胞系AHH-1中S100A4基因的表达变化情况,探讨其基因表达变化的剂量和时间效应关系.方法 永生化淋巴细胞系AHH-1接受0、1、3、5、8、10、15、18 Gy 60Co γ射线照射后4、8、12、24、48和72 h,利用反转录聚合酶链反应(PCR)以及实时荧光PCR技术检测细胞S100A4基因表达水平,分析各时间点其基因的相对表达水平与不同照射剂量之间的关系.结果 照射后各时间点,一定剂量范围内,AHH-1中S100A4基因的表达水平上调,与照射剂量存在着一定的剂量-效应关系(R2=0.79~0.93,P<0.05);在一定时间范围内,AHH-1中S100A4基因表达水平的上调受照射后时间的影响(F=8.91,P<0.01).结论 在一定剂量范围内,辐射诱导的S100A4基因表达在转录水平的变化检测便捷,具有较好的剂量-效应关系,初步具备作为生物剂量计的基本条件. |
| 英文摘要: |
| Objective To investigate the dose response of S100A4 gene expression in the irradiated lymphoblastoid cells AHH-1 at different time points post irradiation. Methods AHH-1 cells was exposed to different doses(0, 1, 3, 5, 8, 10, 15 and 18 Gy)of 60Co γ-rays,and its mRNA levels of S100A4 was detected by reverse transcription PCR and real-time PCR at 4, 8, 12, 24, 48 and 72 h after irradiation. Results Within the range of applied doses, the level of S100A4 gene expression was up-regulated with a good dose-response (R2 =0.79-0.93, P<0.05)and had obvious difference at different time points (F=8.91, P<0.01). Conclusion S100A4 gene expression at transcriptional level could be detected easily and had optimum dose-responses at certain time points after irradiation, and hence is applicable as a dosimeter. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|