中华放射医学与防护杂志

邵立虹,金霖霖,左斯尧,等.辐射对肿瘤微环境中Treg细胞及其相关因子的影响[J].中华放射医学与防护杂志,2015,35(4):241-244.Shao Lihong,Jin Linlin,Zuo Siyao,et al.Effects of the microenvironment of irradiated tumor cells on Treg cells and related regulation factors[J].Chin J Radiol Med Prot,2015,35(4):241-244

辐射对肿瘤微环境中Treg细胞及其相关因子的影响

Effects of the microenvironment of irradiated tumor cells on Treg cells and related regulation factors

投稿时间：2014-11-06

DOI：10.3760/cma.j.issn.0254-5098.2015.04.001

中文关键词: 电离辐射 A549细胞系肿瘤微环境 Treg细胞免疫耐受

英文关键词:Radiation A549 cell line Tumor microenvironment Regulatory T cells Immune tolerance

基金项目:国家自然科学基金(30870584,81371890);教育部高等学校博士学科点专项科研基金(20120061110063)

作者	单位	E-mail
邵立虹	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
金霖霖	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
左斯尧	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
魏巍	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
董卓	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
张海芹	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
韩海玲	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
高辉	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
金顺子	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室	jinsz@jlu.edu.cn

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中文摘要:

目的检测照射后与肺腺癌A549细胞共培养的淋巴细胞中调节性T细胞(Treg细胞)及其相关细胞因子和共刺激分子表达的变化,探讨辐射对肿瘤微环境中免疫耐受机制的影响.方法分离人外周血淋巴细胞单独培养或与肺腺癌A549细胞进行共培养一段时间后,以6 Gy X射线进行照射,继续共培养一段时间后,以流式细胞术检测淋巴细胞中CD4+CD25+、CD4+CD25+FoxP3⁺、CD4+CD25+NrP1⁺不同亚组的百分数、共刺激分子ICOS和CTLA-4的表达情况;ELISA法检测培养体系上清中IL-10、TGF-β、IL-17的分泌量.结果与照射前后单独培养组相比,相应条件下共培养组中CD4+CD25+、CD4+CD25+FoxP3⁺、CD4+CD25+NrP1⁺、ICOS、CTLA-4、IL-10和TGF-β比例均增加(t=2.78~40.61,P<0.05).而与照射前相比,照射后单独培养组和共培养组的CD4+CD25+和CTLA-4比例增加(t=2.96、2.94、4.47、2.77,P<0.05).细胞因子IL-17的表达量在肺腺癌A549细胞和X射线分别作用下无明显变化,但共同作用后会明显增加(t=4.00、4.71,P<0.05).结论辐射刺激肺癌细胞肿瘤微环境中的CD4+CD25+Treg细胞亚组及其相关CTLA-4和IL-17的表达,诱导肿瘤微环境中的免疫耐受作用.

英文摘要:

Objective To explore the immune tolerance in tumor microenvironment, we detected the expressions of CD receptors and related cytokines in Treg cells after being co-cultured with irradiated lung cancer cells. Methods Lymphocytes were isolated from human peripheral blood and cultured with lung adenocarcinoma cells A549, after irradiation with 6 Gy X-rays, the expression of CD4+CD25+, CD4+CD25+FoxP3⁺, CD4+CD25+NrP1⁺and ICOS, CTLA-4 in the lymphocytes were tested by flow cytometry,and the contents of IL-10, TGF-β and IL-17 in the supernatant were detected by ELISA. Results Compared with lymphocytes alone culture, the expressions of CD4+CD25+, CD4+CD25+FoxP3⁺, CD4+CD25+NrP1⁺, ICOS, CTLA-4, IL-10 and TGF-β in the co-culture groups with and without irradiation were all increased (t=2.78-40.61,P<0.05), the CD4+CD25+ and CTLA-4 in the lymphocytes were increased after irradiation (t=2.96,2.94,4.47,2.77,P<0.05). The content of IL-17 in the supernant were increased after co-cuture with irradiated A549 cells (t=4.00,4.71,P<0.05), but it had no obvious changes after irradiation alone. Conclusions The immune tolerance of lung cancer cells microenvironment under irradiation might contribute to the up-promotion of CD4+CD25+ Treg cells, CTLA-4 and IL-17 although the molecular mechanisms still need to be further investigated.

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