| 周睿卿,何慧清,郭子文,牛晓敏,李卫华,邱大发,许晓军,王静.X射线照射对小鼠辅助性T细胞相关细胞因子的影响[J].中华放射医学与防护杂志,2015,35(3):183-186 |
| X射线照射对小鼠辅助性T细胞相关细胞因子的影响 |
| Effects of X-irradiation on the T-helper cell related cytokines in mouse |
| 投稿时间：2014-04-14 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.03.005 |
| 中文关键词: X射线 辅助性T 细胞 细胞因子 干扰素-γ 白介素-17 |
| 英文关键词:X-rays T-helper cells Cytokines IFN-γ IL-17 |
| 基金项目:广东省科技计划项目[粤科社字(2011)106号];中山市科技计划重大专项(20113A002);中山市科技计划(20122A111)资助项目 |
|
| 摘要点击次数: 2563 |
| 全文下载次数: 2185 |
| 中文摘要: |
| 目的 观察不同剂量X射线照射对小鼠辅助性T细胞相关细胞因子的影响。方法 100只BALB/c小鼠用随机数字表法分为4组,包括健康对照组和X射线照射组(分别予2、4和6 Gy 照射),每组25只。于照射前、照射后7、14、21和28 d,对小鼠一般情况进行观察,对外周血进行白细胞计数,用蛋白芯片检测血清中辅助性T细胞相关细胞因子含量,并用ELISA确证细胞因子的浓度变化。结果 照射后,小鼠外周血白细胞14 d降至最低, 同一时间点的下降程度随照射剂量增加而增加 (F=86.267,P<0.05)。芯片法初筛提示,细胞因子IFN-γ、TGF-β、IL-6、IL-17上调,IL-5、IL-23、TNF-α下调。ELISA检测可见,IL-17浓度随辐射剂量增高而明显增高,6 Gy组在7、14和21 d均明显高于对照(t=23.743、21.759和17.662,P<0.05);IFN-γ/IL-4浓度在2和4 Gy照射后无明显变化,6 Gy照射后在7、14、21和28 d均比健康对照组明显增高(t=8.335、9.982、6.990和3.074,P<0.05),呈先升高后降低的变化趋势,14 d达高峰。结论 低于致死剂量的X射线照射可使小鼠血清IFN-γ、TGF-β、IL-6、IL-17细胞因子浓度升高,呈Th1细胞偏移。 |
| 英文摘要: |
| Objective To study the effects of X-ray irradiation at different doses on the expressions of the T-helper cell related cytokines in mouse.Methods 100 BALB/c mice were randomly divided into four groups (25 mice in each) with 0, 2, 4 and 6 Gy X-ray irradiation. At 1, 7, 14, 21 and 28 d after irradiation, the general situation of mice were observed, and the peripheral white blood cells were counted. The alterations of the serum helper T cell related cytokines were screened by protein chips and tested by ELISA.Results At 14 d after irradiation, the peripheral white blood cells of mice decreased to the lowest level. The number of peripheral white blood cells decrease along with the irradiation dose (Fdose=86.267,P<0.05). There were significant increases in the release of IFN-γ, TGF-β, L-6 and IL-17, but decreases in cytokine IL-5, IL-23 and TNF-α. ELISA analysis revealed that the increases of IL-17 correlated with the irradiation dose positively and the concentration of IL-17 in 6 Gy group were higher than control group at 1, 7, 14 and 21 d after irradiation (t=23.743, 21.759 and 17.662,P<0.05). The ratio of IFN-γ/IL-4 significantly higher than the control group at 1, 7, 14, 21 and 28 d after irradiation (t=8.335, 9.982, 6.990 and 3.074,P<0.05), and it approached to the highest level at 14 d and then began to decrease.Conclusions A low dose X-irradiation induces T-helper cell related cytokines IFN-γ and IL-17, and leads to Th1-biased immune responses in mice in vivo. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|