| 徐华林,孙阳,崔凤梅,涂彧.硫酸镁抑制经辐射后人脐静脉血管内皮细胞γ-H2AX的表达[J].中华放射医学与防护杂志,2015,35(3):161-164 |
| 硫酸镁抑制经辐射后人脐静脉血管内皮细胞γ-H2AX的表达 |
| MgSO4 inhibited X-rays irradiation induced expression of γ-H2AX in HUVEC |
| 投稿时间：2014-09-23 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.03.001 |
| 中文关键词: 硫酸镁 人脐静脉血管内皮细胞 X射线 CCK-8 γ-H2AX |
| 英文关键词:Magnesium sulfate Human umbilical vein endothelial X-rays CCK-8 γ-H2AX |
| 基金项目:国家自然科学基金(81372921) |
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| 中文摘要: |
| 目的 探讨不同浓度硫酸镁对照射后人脐静脉血管内皮细胞(human umbilical vein endothelial,HUVEC)存活率及γ-H2AX表达的影响。方法 CCK-8法检测不同浓度的硫酸镁对HUVEC存活率的影响;激光共聚焦显微镜检测4 Gy X射线照射后不同时间HUVEC中γ-H2AX簇集点(foci)的数量;流式细胞术和Western blot检测γ-H2AX蛋白的表达情况。结果 CCK-8法显示,1.25 mg/ml浓度的硫酸镁能提高照射后的细胞存活率(t=-6.34,P<0.05);细胞免疫荧光结果显示,照射后0.5~1 h foci焦点数达到最高值,平均达45个,随着时间的延长foci点数变少,强度减弱,具有时间依赖性,而硫酸镁在照后0.5、1、2、6、12 h均可以明显减少foci的形成(t=12.62、6.36、11.93、5.75、9.43,P<0.05);流式细胞仪检测表明,硫酸镁在照后0.5、1、2 h可以抑制X射线照射后γ-H2AX蛋白表达量的增加(t=6.07、5.32、11.85,P<0.05);Western blot结果与细胞免疫荧光结果一致。结论 硫酸镁可以增加照射后HUVEC的存活率,降低X射线诱导的DNA损伤蛋白γ-H2AX的表达。 |
| 英文摘要: |
| Objective To investigate the influence of MgSO4 with different concentrations on cell survival and γ-H2AX expression in HUVEC irradiated with X-rays.Methods Cell proliferation rate was assayed by CCK-8, γ-H2AX foci formation was observed with a laser confocal microscope, and γ-H2AX protein expression was detected by flow cytometer and Western blot assay.Results MgSO4 with a concentration of 1.25 mg/ml could improve the survival rate of HUVEC treated with X-rays (t=-6.34,P<0.05).After irradiation, γ-H2AX foci approached to the highest level from 30 min to 1 h after radiation and then decreased. MgSO4 significantly reduced the foci formation at 0.5, 1, 2, 6 and 12 h post-irradiation (t=12.62, 6.36, 11.93, 5.75, 9.43,P<0.05). Both flow cytometry and Western blot assays showed that MgSO4 inhibited γ-H2AX protein expression at 0.5, 1 and 2 h post-irradiation (t=6.07, 5.32, 11.85,P<0.05).Conclusions MgSO4 could improve the survival rate and reduced γ-H2AX expression in HUVEC irradiated with X-ray. |
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