| 刘红艳,党旭红,刘建功,张慧芳,原雅艺,王超,左雅慧,段志凯.12C重离子束对人淋巴细胞增殖、周期和凋亡的影响[J].中华放射医学与防护杂志,2015,35(2):107-110 |
| 12C重离子束对人淋巴细胞增殖、周期和凋亡的影响 |
| Effects of 12C heavy-ion irradiation on cell growth, cell cycle and apoptosis of human peripheral blood lymphocytes |
| 投稿时间：2014-05-20 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.02.007 |
| 中文关键词: 12C重离子 人淋巴细胞 细胞增殖 细胞周期 细胞凋亡 |
| 英文关键词:12C heavy-ions Human peripheral blood lymphocytes Cell proliferation Cell cycle Cell apoptosis |
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| 中文摘要: |
| 目的 探讨12C重离子束对人淋巴细胞增殖以及周期、凋亡的影响.方法 12C重离子束照射人淋巴细胞Peng-EBV,吸收剂量分别为0(对照组)、0.5、2.0 Gy.照射后用MTS法检测细胞增殖活力,流式细胞仪检测细胞周期和细胞凋亡.结果 与对照组相比,0.5 Gy照射可以增加细胞增殖活力(t=2.66~14.45, P<0.05),而2.0 Gy照射降低了细胞活力(t=7.65~64.45, P<0.05).受照射细胞的活力存在一个恢复和下降过程,受照后48 h内,细胞数量呈增加趋势,但72 h时细胞数量下降.照射后48 h,两组细胞G2/M期呈明显上升趋势,高于对照组(t=2.01~99.80,P<0.05),且2.0 Gy组的周期阻滞较0.5 Gy组严重;照射后30 d,细胞周期阻滞恢复到正常水平.照射后12、24、48 h,两照射组与对照组相比,细胞凋亡率差异有统计学意义(t=-3.05~-1.05,P<0.05),在照后24 h最高,48 h明显下降,30 d恢复到对照水平.结论 12C离子束辐射影响人淋巴细胞增殖,诱导人淋巴细胞发生明显的G2/M期阻滞,并且明显地促进细胞凋亡. |
| 英文摘要: |
| Objective To explore the effects of 12C ions beam exposure on cell growth, cell cycle and apoptosis of lymphocytes.Methods Human peripheral blood lymphocytes Peng-EBV were exposed to 12C ions beams with different dose (0, 0.5, 2.0 Gy) at the dose rate of 0.3-0.5 Gy/min. The proliferation of Peng-EBV cells was determined by MTS assay. Cell apoptosis and cell cycle were analyzed by flow cytometry.Results The cellular proliferation was enhanced by 0.5 Gy irradiation but reduced by 2.0 Gy irradiation. The proliferation of irradiated cells could be recovered at 48 h after irradiation, but decreased at 72 h post irradiation. After 48 h of irradiation, G2/M cell cycle arrest was caused, especially at 2.0 Gy (t=2.01-99.80, P<0.05). At 30 d after irradiation, G2/M cell cycle arrest was not detected. At 12, 24, 48 h post-irradiation, apoptotic level was enhanced compared with the control (t=-3.05-1.05, P<0.05). The apoptosis rate had the highest level at 24 h post-irradiation, and then dropped at 48 h post-irradiation and recovered to control at 30 d post-irradiation.Conclusions The heavy-ion beam exposure could affect cellular proliferation, increase apoptosis and induce G2/M phase arrest of lymphocytes. |
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