| 王彦,杜利清,徐畅,等.新型Smac模拟物Tat-Smac N7的肿瘤细胞辐射增敏作用[J].中华放射医学与防护杂志,2015,35(1):53-56.Wang Yan,Du Liqing,Xu Chang,et al.Radiosensitization in tumor cells induced by Tat-Smac N7 fusion peptide[J].Chin J Radiol Med Prot,2015,35(1):53-56 |
| 新型Smac模拟物Tat-Smac N7的肿瘤细胞辐射增敏作用 |
| Radiosensitization in tumor cells induced by Tat-Smac N7 fusion peptide |
| 投稿时间：2014-10-02 |
| DOI：10.3760/cma.j.issn.0254-5098.2015.01.010 |
| 中文关键词: Smac蛋白 EC109 H460 细胞凋亡 辐射敏感性 |
| 英文关键词:Smac protein EC109 H460 Cell apoptosis Radiosensitivity |
| 基金项目:国家自然科学基金(31170804);天津市自然科学基金(12JCYBJC15300,13JCYBJC23500) |
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| 中文摘要: |
| 目的 观察Tat-Smac N7融合肽对人肺癌细胞系H460和人食管癌细胞系EC109的辐射增敏作用,探讨其辐射增敏机制. 方法 取对数生长期H460和EC109细胞,分为DAPI对照组、FITC-Smac N7和FITC-Tat-Smac N7组,荧光显微镜观察两种细胞不同时间药物入核情况.取对数生长期H460和EC109细胞,分为单纯照射组、照射联合Tat-Smac N7组,单纯照射组给予0、2、4、6 Gy照射,照射联合Tat-Smac N7组中Tat-Smac N7的浓度为20 μmol/L,WST-1测定Tat-Smac N7的辐射增敏作用.取对数生长期H460和EC109细胞,分为对照组、Tat-Smac N7组、单纯照射组和照射联合Tat-Smac N7组,吸收剂量为4 Gy,Tat-Smac N7浓度为20 μmol/L,细胞流式分析仪测定细胞不同时间的细胞凋亡率.结果 Tat-Smac N7融合蛋白进入2种细胞系后2 h可以有蓄积,且这种蓄积可延续到24 h,而Smac N7则不能进入细胞.Tat-Smac N7能够增强H460和EC109细胞的辐射敏感性(F=22.2、13.2,P<0.05),照射联合Tat-Smac N7可明显增加辐射诱导的细胞凋亡率(24 h:F=9.32、5.86,P<0.05;48 h:F=7.09、8.25,P<0.05).Tat-Smac N7联合照射后凋亡诱导效应具有时间依赖性.结论 Tat-Smac N7融合肽可促进肿瘤细胞的辐射敏感性,作为一种新的Smac蛋白类似物,有望用于肿瘤的辐射增敏治疗. |
| 英文摘要: |
| Objective To observe the radiosensitization effect of Tat-Smac N7 fusion peptide in EC109 and H460 cell lines, and to explore the mechanism of Tat-Smac N7 in radio sensitization.Methods H460 and EC109 cells were divided into DAPI group, and FITC-Smac N7 group, FITC-Tat-Smac N7 group. Fluorescence microscope was used to detect if the peptide had been entered into tumor cells at deferent times. H460 and EC109 cells were divided into radiation group and Tat-Smac N7 combined with radiation group. The cells were irradiated with 4 Gy γ-ray and the concentration of Tat-Smac N7 was 20 μmol/L. The proliferation of tumor cells was detected by WST-1 assay. Among control group, radiation group, Tat-Smac N7 group and Tat-Smac N7 combined with radiation group, apoptosis was detected by flow cytometry. Results Tat-Smac N7 could enter cells for 2-24 h, but Smac N7 couldn't. Tat-Smac N7 promoted the radio sensitization of H460 and EC109 cells obviously (F=22.2, 13.2,P<0.05). The apoptosis of combined group was much higher than that of control (24 h: F=9.32, 5.86, P<0.05; 48 h: F=7.09, 8.25, P<0.05). The apoptosis in Tat-Smac N7 combined with radiation group varied with time-dependence. Conclusions Tat-Smac N7 fusion peptide showed remarkable radiosensitization in tumor cells. As a new Smac mimetic, Tat-Smac N7 was potential in radio sensitization therapy of tumor in the future. |
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