| 王越,鲁纯雪,常晨旭,等.微波辐射对下丘脑室旁核组织结构及功能的影响[J].中华放射医学与防护杂志,2026,46(9):811-817.Wang Yue,Lu Chunxue,Chang Chenxu,et al.Effects of microwave radiation on the histological structure and function of the hypothalamic paraventricular nucleus[J].Chin J Radiol Med Prot,2026,46(9):811-817 |
| 微波辐射对下丘脑室旁核组织结构及功能的影响 |
| Effects of microwave radiation on the histological structure and function of the hypothalamic paraventricular nucleus |
| 投稿时间：2025-11-27 |
| DOI：10.3760/cma.j.cn112271-20251127-00414 |
| 中文关键词: 微波辐射 下丘脑室旁核 应激反应 细胞因子 前脉冲抑制 |
| 英文关键词:Microwave Hypothalamic paraventricular nucleus Stress response Cytokine Prepulse inhibition |
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| 中文摘要: |
| 目的 研究微波辐射对机体应激反应、血清细胞因子及下丘脑室旁核（PVN）组织结构及功能的影响，探讨微波辐射致机体应激反应的调节机制。方法 选取C57BL/6N小鼠54只，按照随机数表法分为对照组和微波辐射组，每组27只。采用频率为2.856 GHz、功率密度为 8 mW/cm2、比吸收率（SAR）为6.12 W/kg的微波全身辐射小鼠15 min，建立微波辐射小鼠模型。通过震惊条件反射前脉冲抑制（PPI）行为学实验，研究微波辐射对小鼠应激反应的影响；采用放射免疫标记法检测血清去甲肾上腺素（NE）、皮质酮（CORT）、促肾上腺皮质激素（ACTH）、促肾上腺皮质激素释放激素(CRH)、神经元特异性烯醇化酶（NSE）、中枢神经特异性β蛋白（S100-β）、脑组织肿瘤坏死因子α（TNF-α）、白介素1β（IL-1β）、IL-4、IL-6、IL-10含量；通过脑组织切片和苏木精-伊红(HE)染色，观察PVN组织病理学变化和超微结构变化。结果 微波照后3 d，微波辐射组小鼠PPI于70、80 dB时均较对照组显著降低，差异均有统计学意义（F=6.11、6.74，P < 0.05）；照后7 d，微波辐射组小鼠血清CORT、NE、ACTH、CRH、NSE、S100-β、TNF-α、IL-1β、IL-6含量均较对照组显著升高，差异均有统计学意义（t= 4.79、3.94、6.89、3.40、11.39、9.96、2.97、3.55、3.81，P < 0.05），IL-4、IL-10未见明显变化(P > 0.05)；照后7 d，微波辐射组小鼠PVN部分神经元核固缩深染，超微结构观察可见神经元核染色质凝聚，胶质细胞线粒体肿胀，突触间隙结构模糊。结论 微波辐射可引起小鼠PPI能力降低，血清应激激素、促炎细胞因子含量升高，PVN组织病理学改变，提示微波辐射可引起PVN组织结构和功能改变，进而影响血清细胞因子水平导致机体应激反应发生。 |
| 英文摘要: |
| Objective To investigate the effects of microwave radiation on stress response, serum cytokines, and the histological structure and function of the hypothalamic paraventricular nucleus (PVN), and to explore the regulatory mechanisms of microwave radiation-induced stress responses.Methods A total of 54 C57BL/6N mice were selected and randomly assigned to a control group and a microwave radiation group, with 27 mice in each group. The mice were exposed to whole-body microwave radiation at a frequency of 2.856 GHz, a power density of 8 mW/cm2, and a specific absorption rate of 6.12 W/kg for 15 min to establish a mouse model of microwave exposure. The effect of microwave exposure on the stress response of mice was studied via the prepulse inhibition behavioral test of the startle reflex. Radioimmunoassay was used to detect the serum levels of norepinephrine, corticosterone, adrenocorticotropic hormone, corticotropin-releasing hormone, neuron-specific enolase, and central nervous system-specific β-protein S100-β, as well as tumor necrosis factor-α, interleukin-1β (IL-1β), IL-4, IL-6, and IL-10. Brain tissue sectioning and hematoxylin-eosin staining were used to observe the histopathological and ultrastructural changes in the PVN.Results At 3 d after microwave exposure, the percentages of prepulse inhibition(PPI) of mice in the microwave radiation group were significantly lower than those in the control group at 70 and 80 dB (F=6.11, 6.74, P<0.05). At 7 d after exposure, the levels of corticosterone, norepinephrine, adrenocorticotropic hormone, corticotropin-releasing hormone, neuron-specific enolase, central nervous system-specific β-protein S100-β, tumor necrosis factor-α, IL-1β, and IL-6 in the microwave radiation group were significantly elevated compared with the control group (t=4.79, 3.94, 6.89, 3.40, 11.39, 9.96, 2.97, 3.55, 3.81, P<0.05), while no significant changes were observed in IL-4 and IL-10 (P>0.05). At 7 d after microwave exposure, some neurons in the PVN of mice in the microwave radiation group exhibited nuclear pyknosis and hyperchromasia. Ultrastructural observations revealed chromatin condensation in neuronal nuclei, mitochondrial swelling in glial cells, and blurred synaptic cleft structures.Conclusions Microwave exposure can reduce the prepulse inhibition in mice, increase the serum levels of stress hormones and pro-inflammatory cytokines, and cause histopathological changes in the PVN, suggesting that microwave radiation can induce changes in the histological structure and function of the PVN, thereby affecting serum cytokine levels and leading to stress response. |
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