| 刘晓丹,张士猛,李兵,尚增甫,徐勤枝,周平坤.60Co γ射线诱导正常人淋巴母细胞PIG3 基因mRNA表达的剂量相关性研究[J].中华放射医学与防护杂志,2011,31(6):623-626 |
| 60Co γ射线诱导正常人淋巴母细胞PIG3 基因mRNA表达的剂量相关性研究 |
| Dose-dependent pattern of inducible mRNA expression of PIG3 gene in normal human lymphoblastoid cells by 60Co γ-rays |
| 投稿时间：2011-03-09 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.06.001 |
| 中文关键词: 放射损伤 生物剂量计 PIG3 DNA损伤 基因表达 实时定量PCR |
| 英文关键词:Radiation injury Biodosimeter PIG3 DNA damage Gene expression Real-Time PCR |
| 基金项目:国家自然科学基金杰出青年基金(30825011) |
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| 中文摘要: |
| 目的 探讨正常人淋巴母细胞AHH1电离辐射作用后PIG3基因mRNA表达变化的剂量-效应规律,建立一种基于基因表达变化的新型辐射生物剂量计的可能性。方法 激光共聚焦检测DNA双链断裂分子标记γ-H2AX集簇点,1、2、4、6、8和10 Gy 60Co γ射线照射AHH1细胞后4、10和24 h收集细胞,应用实时荧光定量PCR技术对PIG3基因mRNA表达水平进行相对定量检测。结果 γ射线照射后30 min检测发现PIG3蛋白与γ-H2AX在细胞核内有部分共定位,形成集簇点(foci),表明其参与电离辐射致DNA双链断裂损伤信号识别反应。γ射线诱导PIG3基因 mRNA表达水平随时间推移不断提高,持续时间可达24 h。PIG3基因mRNA表达水平具有显著的剂量依赖性,其表达丰度变化的剂量依赖范围在照射后4 h为0~ 6 Gy、照射后10和24 h均为0~10 Gy。结论 PIG3基因参与DNA双链断裂损伤信号识别反应,其mRNA的辐射诱导表达具有良好的剂量-效应关系,具有发展成为新的放射生物剂量计的价值。 |
| 英文摘要: |
| Objective To investigate the dose-response pattern on the inducible expression of PIG3 mRNA in normal human lymphoblastoid AHH1 cells by 60Co γ-rays, and its possibility for developing novel radiation biodosimeter. Methods Laser confocal fluorescent microscopy was used to detect the γ-H2AX foci, a biomarker of DNA double-strand break. After irradiation with 0,1,2,4,6,8 and 10 Gy of 60Co γ-rays, AHH-1 cells were harvested at 4, 10 and 24 h post-irradiation, and subjected to total RNA extraction and detection of PIG3 mRNA expression by quantitative real-time PCR. Results PIG3 protein foci were formed in the nuclei at 30 min after irradiation, and a part of these PIG3 foci were colocalized with γH2AX foci. After irradiation, PIG3 mRNA level was enhanced with the increased time of post-irradiation, and remained an increased level at least till 24 h. The radiation-inducible expression of PIG3 mRNA was demonstrated in a dose-dependent manner. The dose-dependent range at 4 h post-irradiation was 0-6 Gy, and at 10 h and 24 h was 0-10 Gy. Conclusions PIG3 involves in the cellular response to DNA double-strand break. The dose-dependent inducible response of PIG3 mRNA expression might provide a valuable candidate for developing a novel radiation biodosimeter. |
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