| 刘丽波,张俭,刘宇光,倪冠英,刘树铮.辐射诱导小鼠外周血网织红细胞微核与骨髓嗜多染红细胞微核的 剂量-效应关系[J].中华放射医学与防护杂志,2011,31(2):122-125 |
| 辐射诱导小鼠外周血网织红细胞微核与骨髓嗜多染红细胞微核的 剂量-效应关系 |
| Correlation analysis on MN-RET from peripheral blood and MN-PCE from bone marrow in mice following exposure to irradiation |
| 投稿时间：2010-12-01 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.02.002 |
| 中文关键词: 辐射 小鼠 外周血网织红细胞微核 骨髓嗜多染红细胞微核 流式细胞术 生物剂量计 |
| 英文关键词:Radiation Mice Reticulocyte micronucleus Polychromatic erythrocyte micronucleus Flow cytometry Biodosimeter |
| 基金项目:卫生行业科研专项(200802018) |
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| 中文摘要: |
| 目的 观察X射线照射后小鼠外周血网织红细胞微核(MN-RET)和骨髓嗜多染红细胞微核(MN-PCE)的变化,为探索快速、高通量的辐射生物剂量计奠定基础。方法 54只ICR雄性小鼠随机分为3组,每组18只,分别施予X射线全身照射,吸收剂量分别为0、0.5、1、2、4和5 Gy。在照射后24、48和72 h,分别采用流式细胞术(FCM)和人工镜检的方法,观察外周血MN-RET和骨髓MN-PCE的变化。结果 在0.5~5.0 Gy范围内,3个时间点外周血MN-RET和骨髓MN-PCE均随剂量的增加而升高,剂量-效应曲线可拟合成直线回归方程( t =10.26~25.77, P ＜0.05);外周血MN-RET和骨髓MN-PCE之间明显相关( r= 0.986~0.996, P ＜0.05)。 结论 FCM检测MN-RET有望成为快速、高通量的辐射生物剂量计或辅助诊断方法。 |
| 英文摘要: |
| Objective To study the changes of reticulocyte micronucleus(MN-RET) from peripheral blood and polychromatic erythrocyte micronucleus(MN-PCE) from bone marrow in mice following exposure to X-rays in order to provide an experimental basis for exploring possible high-throughput radiation biodosimeter. Methods Male ICR mice were whole-body irradiated with 0, 0.5, 1, 2, 4 and 5 Gy at a dose rate of 0.488 Gy/min. MN-RET from peripheral blood was scored with FCM and MN-PCE from bone marrow was scored with manual microscopy at 24, 48 and 72 h post-irradiation. Results Both MN-RET and MN-PCE rates increased with doses in the range of 0-5 Gy at 24, 48 and 72 h after WBI. The dose-response relationship can be fit with linear equations (t=10.26-25.77, P<0.05). The correlation coefficients between MN-RET from peripheral blood and MN-PCE from bone marrow were highly significant (r=0.986-0.996, P<0.05). Conclusions In view of its simplicity, accuracy and high-throughput capacity, FCM scoring of peripheral blood MN-RET may be a candidate for radiation biodosimetry. More work should be carried out on human specimens to investigate this possibility. |
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