中华放射医学与防护杂志

郑辉,甄荣,涂序珉,张志兴.辐射损伤后小鼠T细胞功能亚群及其IL-4和IFN-γ的变化[J].中华放射医学与防护杂志,2009,29(4):386-388

辐射损伤后小鼠T细胞功能亚群及其IL-4和IFN-γ的变化

Changes of T-lymphocyte function subsets and IL-4 and IFN-γ after irradiation injury

投稿时间：2008-09-28

DOI：

中文关键词: 辐射免疫损伤 T 淋巴细胞亚群白细胞介素-4(IL-4) IFN-γ

英文关键词:Radiation Immune Harm T-lymphocyte subsets IL-4 IFN-γ

基金项目:

作者	单位
郑辉	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
甄荣	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
涂序珉	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
张志兴	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所

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中文摘要:

目的观察不同剂量辐射损伤后小鼠T细胞功能亚群、细胞因子IL-4和IFN-γ的变化。方法 C57BL/6j小鼠分为假照射组和辐射损伤模型组。采用⁶⁰Coγ线照射诱导辐射损伤模型。吸收剂量分别为0.7、1.4、2.8及5.6 Gy。应用细胞表面标志和细胞内因子标记结合流式细胞仪分析急性照射损伤和损伤恢复期小鼠脾CD3+、CD4+、CD8+T细胞功能亚群,以及细胞因子IL-4和IFN-γ的变化。结果 1 辐射损伤后CD3+、 CD4+及 CD8+有明显的减低,其改变幅度与受照剂量有关。2 照射后1 d, IFN-γ降低幅度明显高于IL-4,受照剂量大于2.8 Gy组,IL-4/IFN-γ比值较空白对照组明显升高。3 辐射对淋巴细胞功能亚群、细胞因子IL-4和IFN-γ的损伤在照射后25 d有不同程度的恢复,但与假照射组比较仍有明显差别。结论电离辐射可使淋巴细胞亚群CD3+,CD4+,CD8+、 CD4+/CD8+、细胞因子IL-4和IFN-γ发生改变,并诱发受照小鼠免疫功能低下,特别是使细胞因子IL-4和IFN-γ发生失衡,再次证实辐射损伤后小鼠脾Th1/Th2模式向Th2免疫反应漂移的现象。

英文摘要:

Objective To observe the effects of T-lymphocyte function subsets,IL-4 and IFN-γ cell factor in different dose and stage after irradiation. Methods The C57BL/6j mice were divided into shammed irradiation group and model groups. The radiation hurt model was induced by ⁶⁰Co gamma rays (0.7, 1.4, 2.8 and 5.6 Gy). The changes of T- lymphocyte subsets CD3,CD4,CD8, IL-4 and IFN-γ in spleen cells were analyzed by flow cytometry in acute injury stage and recovery stage after irradiation. Results The lymphocyte subsets CD3+, CD4+ and CD8+ decreased after irradiation,which were related to the irradiation dose. At 1 day after irradiation, the decreasing level of IFN-γ was higher than that of IL-4. When irradiation dose was over 2.8 Gy, IL-4 / IFN-γ showed a markedly increased compared with control group. At 25 days after irradiation, CD3+,CD4+,CD8+,CD4+/CD8+,IL-4 and IFN-γ recovered obviously, but they did not recover to the normal level of shammed irradiation group. Conclusions The depression of mouse immune function induced by γ-irradiation might be caused by changes of CD3,CD4,CD8 and CD4/CD8 ratio, especially the imbalance of IL-4 and IFN-γ.

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