| 于洋,曹慧玲,徐冶,刘师兵.2.45GHz微波非热效应诱导人宫颈癌细胞凋亡及其机制[J].中华放射医学与防护杂志,2014,34(12):908-911 |
| 2.45GHz微波非热效应诱导人宫颈癌细胞凋亡及其机制 |
| The mechanism of non-thermal effect of 2.45 GHz microwave on HeLa cell apoptosis |
| 投稿时间：2014-06-10 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.12.007 |
| 中文关键词: 微波 非热效应 HeLa细胞 细胞凋亡 |
| 英文关键词:Microwave Non-thermal effect HeLa cells Cell apoptosis |
| 基金项目:吉林省教育厅"十一五"科学技术研究(吉教科合字[2010]第253号) |
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| 中文摘要: |
| 目的 观察2.45 GHz微波非热效应对人宫颈癌HeLa细胞凋亡的影响,探讨不同照射功率与凋亡诱导效应之间是否存在量效关系及其机制.方法 HeLa细胞随机分为对照组、假照射组和低、中、高(10、15和20 mW/cm2)3个功率照射组,2.45 GHz微波照射10 min.MTT法检测HeLa细胞存活率;激光共聚焦显微镜观察各组HeLa细胞核形态变化,并统计异常细胞核比率;流式细胞仪检测细胞周期并分析sub-G1期细胞所占比例;免疫印迹法观察Bax、Bcl-2和Caspase-3凋亡相关蛋白表达变化并以β-肌动蛋白为内参比较各组蛋白表达灰度变化.结果 MTT结果显示,对照组与假照射组、低功率照射组之间细胞存活率无明显变化,中、高功率照射组HeLa细胞存活率较假照射组明显降低(t=-16.47、-14.23,P<0.05);激光共聚焦观察经Hoechst单染的细胞核显示,低、中、高功率照射组异常细胞核比率与假照射组相比明显增高(t=9.37、11.25、8.47,P<0.05),且高功率组细胞核异常比率高于低、中功率组(t=15.32、10.25,P<0.05);流式细胞术结果显示低、中、高功率照射组sub-G1期细胞所占比例均比假照射组升高(t=15.24、22.31、10.69,P<0.05);免疫组织化学结果显示Bcl-2的表达随着微波照射功率的增大而减弱,Bax和Caspase-3的表达随着照射功率的增强而加强.结论 2.45 GHz微波可诱导HeLa细胞凋亡,且与其功率密度增加呈正相关. |
| 英文摘要: |
| Objective To investigate the effects of 2.45 GHz microwave on the apoptosis of HeLa cells and explore the underlying mechanism.Methods HeLa cells were divided into five groups randomly: control group, sham-irradiation group, and three irradiation groups exposed with 10,15 and 20 mW/cm2 2.45 GHz microwave for 10 min. Cell survival was tested by MTT assay, cell nucleus fashion was observed by a laser scanning confocal microscope, cell cycle distribution was measured by flow cytometry, and cell protein expressions were detected by Western blot. Results The cell survival in control group, sham-irradiation group and low dose irradiation group had no difference but it became lower in the middle and high dose irradiated group(t=-16.47, -14.23, P<0.05). The abnormal rates of nuclear fashion in the low,middle and high dose irradiation groups were obviously higher compared with control group(t=9.37, 11.25, 8.47, P<0.05), while high dose irradiation group was higher than the low and middle dose group (t=15.32, 10.25, P<0.05). The rate of HeLa cells in sub-G1 in the irradiation groups were higher than that in control group(t=15.24, 22.31, 10.69, P<0.05). The results of immunohistochemistry showed that the expression of Bax and Caspase-3 in irradiation groups was significantly increased, and the expression of Bcl-2 was decreased. Conclusions 2.45 GHz microwave can induce HeLa cells apoptosis with a positive correlation with microwave dose. |
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