| 王晶,万文丽,张巍,董菲,朱明霞,汪整辉,刘建香,苏旭,克晓燕.60Coγ照射和环磷酰胺对小鼠造血及免疫功能影响的研究[J].中华放射医学与防护杂志,2014,34(12):904-907 |
| 60Coγ照射和环磷酰胺对小鼠造血及免疫功能影响的研究 |
| Effects of ionizing radiation and cyclophosphamide on hematopoietic and immune functions in mice |
| 投稿时间：2014-04-14 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.12.006 |
| 中文关键词: 急性放射综合征 化疗 造血功能 免疫功能 |
| 英文关键词:Acute radiation syndrome, ARS Chemotherapy Hematopoietic function Immunologic function |
| 基金项目:卫生行业科研专项项目(201002009) |
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| 中文摘要: |
| 目的 比较60Co γ射线照射及环磷酰胺对小鼠造血和免疫功能损伤的规律,为急性放射综合征(ARS)患者临床救治提供实验依据.方法 48只雌性BABL/c小鼠随机数字表法分为照射组、化疗组,每个剂量组6只.照射组接受60Co γ射线照射,吸收剂量为2、4、6和8 Gy;化疗组注射环磷酰胺,剂量为100、150、200和250 mg/kg.处理后采尾静脉血,进行血常规检测.选择白细胞(WBC)差异无统计学意义的2 Gy和200 mg/kg组,处理后流式细胞术检测T、B、NK及调节T细胞.结果 各组外周血WBC计数均先下降,4 d后降至最低值.化疗组和2 Gy照射组WBC迅速回升,10~12 d基本恢复;而4 Gy照射组恢复较慢,6和8 Gy组不能自行恢复.2 Gy照射组与200 mg/kg化疗组差异无统计学意义,其余照射组与200 mg/kg化疗组的差异有统计学意义(t=2.531, 2.343, 2.074, P<0.05).2 Gy照射组CD8+T细胞数较200 mg/kg化疗组明显下降(F=5.026,P<0.05),2 Gy照射组调节T细胞在照射后升高,与200 mg/kg化疗组比较差异有统计学意义(F=4.848,P<0.05),但CD4+T、B和NK细胞数两组之间差异无统计学意义.结论 电离辐射较化疗对造血、免疫功能的损伤较大,恢复较慢.因此,在ARS患者中可针对性延长造血刺激因子和集落刺激因子的使用,以起到预防感染、出血及败血症、帮助造血及免疫系统重建的作用. |
| 英文摘要: |
| Objective To compare the injury and recovery effects of ionizing radiation and chemotherapy on hematopoietic and immunologic functions in mice and provide the experimental evidence for clinical treatment of acute radiation syndrome (ARS).Methods Mice were divided into radiation groups exposed to 2, 4, 6 and 8 Gy of 60Co γ-rays and chemotherapy groups administered with cyclophosphamide (CTX) at dosages of 100, 150, 200 and 250 mg/kg. Blood routine examination was performed in all groups. According to the pilot result of blood routine examination, 2 Gy group and 200 mg/kg group were selected to analyze the immunocytes subsets (T cells, B cells, NK cells and Tregs).Results In each group, the number of white blood cells (WBC) in peripheral blood decreased and dropped to a minimum level at 4 d. The chemotherapy and 2 Gy group quickly rebounded to restore at 10-12 d, but 4 Gy group recovered more slowly. There was no significantly different between 2 Gy group with 200 mg/kg chemotherapy group and there was significantly different between other doses of radiation groups with chemotherapy groups (t=2.531, 2.343, 2.074, P<0.05). The numbers of CD8+ T cells were decreased significantly in irradiated group than chemotherapy group(F=5.026, P<0.05). The number of regulator T cells (Tregs) were higher obviously increased in irradiated group, and decreased in chemotherapy group, the difference was statistically significant (F=4.848, P<0.05). But the number of CD4+ T cells, B cells and NK cells were no significant differences between radiation group and chemotherapy group. Conclusions The recovery of immunocytes in ionizing radiation group was slower than that in chemotherapy group. Chemotherapy had less effect of immune suppression and damage compared with ionizing radiation. Delay of immunologic reconstitution is the main cause of ARS death to get effective therapy result, the application of hematopoietic stimulating factor may be necessary. |
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