| 高玲,刘巧维,李峰生,等.γ射线诱导人肺腺癌H322细胞STAT3激活的实验研究[J].中华放射医学与防护杂志,2014,34(12):900-903.Gao Ling,Liu Qiaowei,Li Fengsheng,et al.Radiation-induced activation of STAT3 in pulmonary adenocarcinoma[J].Chin J Radiol Med Prot,2014,34(12):900-903 |
| γ射线诱导人肺腺癌H322细胞STAT3激活的实验研究 |
| Radiation-induced activation of STAT3 in pulmonary adenocarcinoma |
| 投稿时间：2014-04-16 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.12.005 |
| 中文关键词: 肺腺癌 辐射 p-STAT3 表皮生长因子受体 |
| 英文关键词:Adenocarcinma Irradiation p-STAT3 EGFR |
| 基金项目:国家自然基金科学部主任基金(31340051);国家自然科学基金青年科学基金(81001216);全军医学科技"十二五"科研项目(CWS12J082) |
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| 中文摘要: |
| 目的 探索辐射诱导人肺腺癌细胞STAT3活化的剂量和时间效应及其上游信号通路.方法 60Co γ射线照射p53突变型肺腺癌细胞H322,0~10 Gy照射后0~24 h提取蛋白,利用Western blot方法和免疫荧光染色实验,检测H322细胞中磷酸化STAT3表达和入核情况,以及其上游信号分子p-EGFR水平.结果 Western blot方法和免疫荧光染色实验均表明2~10 Gy照射后(1.9~6.6倍)0~24 h(1.2~9.5倍)均可诱导肺腺癌细胞H322中p-STAT3表达增高;同时发现H322细胞中出现p-EGFR表达的增加,其为对照组的4.3~19.2倍.结论 60Co γ射线照射后引起肺腺癌H322细胞中p-STAT3表达增高,STAT3的激活可能由EGFR/STAT3信号通路所介导. |
| 英文摘要: |
| Objective To investigate the dose-, time-effect of STAT3 activation induced by radiation and explore the underlying signal pathway. Methods Pulmonary adenocarcinoma cells (H322) with mutated p53 gene were irradiated with 0-10 Gy of γ-rays. After radiation, the expression and transcriptional activity of STAT3 and the levels of its upstream molecules, including p-EGFR, were detected by Western blot. The nucleus translocation of p-STAT3 was examined by immunofluorescence staining assay. Results The protein expression and transcriptional activation of p-STAT3 in H322 cells were increased by 1.9-6.6 fold after 2-10 Gy radiation and increased by 1.2-9.5 fold after 0-24 h of irradiation. In addition, the expression of p-EGFR was also increased by 4.3-19.2 fold after irradiation. Conclusions After radiation, the expression of p-STAT3 in the pulmonary adenocarcinoma H322 cell can be increased probably through EGFR/STAT3 signal pathway. |
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