| 程义壮,姚晓波,吴翠萍,王永生,陈柯,张伍魁,王明明.18F-FDG对小鼠Lewis肺癌移植瘤P53、XIAP与GADD45蛋白表达的影响[J].中华放射医学与防护杂志,2014,34(12):884-888 |
| 18F-FDG对小鼠Lewis肺癌移植瘤P53、XIAP与GADD45蛋白表达的影响 |
| Effects of 18F-FDG on P53, XIAP, and GADD45 expressions in transplanted Lewis lung carcinoma in mice |
| 投稿时间：2014-03-28 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.12.002 |
| 中文关键词: Lewis肺癌 P53蛋白 XIAP蛋白 GADD45蛋白 |
| 英文关键词:Lewis lung carcinoma P53 protein XIAP protein GADD45 protein |
| 基金项目:国家自然科学基金(81273003);安徽省自然科学基金(1208085MH162);安徽省科技攻关项目(10021303035);安徽省教育厅自然科学基金(KJ2011Z162) |
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| 中文摘要: |
| 目的 观察18F-氟代脱氧葡萄糖(18F-FDG)内照射对Lewis肺癌移植瘤P53、XIAP与GADD45蛋白表达的影响,探讨其作为内照射治疗药的可行性.方法 建立Lewis肺癌C57BL/6小鼠皮下移植瘤模型48只,随机数字表法分为高剂量组、低剂量组和对照组,每组 16只.接种后第7天,3组小鼠分别腹腔注射0.2 ml 18F-FDG及等体积生理盐水,使高、低剂量组18F-FDG注射量分别为18.5×107和9.25×107 Bq.第22天处死小鼠,SP免疫组织化学法检测移植瘤组织P53、XIAP与GADD45蛋白表达.结果 每种蛋白3组小鼠间表达差异有统计学意义(χ2=8.30、16.02、7.68,P<0.05).3组小鼠Lewis肺癌P53蛋白表达的平均积分光密度分别从对照组的(0.089±0.033)随照射剂量增大为高剂量组的(0.315±0.028);XIAP蛋白表达的平均积分光密度从(0.422±0.034)逐步减小为(0.149±0.055);GADD45蛋白表达的平均积分光密度从(0.126±0.023)逐步上升为(0.383±0.035).3组间蛋白表达量差异有统计学意义(P53:F=5.26,P<0.05;XIAP:F=4.29,P<0.05;GADD45:F=5.98,P<0.05).结论 18F-FDG能够上调P53和GADD45蛋白表达,下调XIAP蛋白表达,促使小鼠Lewis肺癌组织细胞以P53依赖的方式凋亡,抑制Lewis肺癌移植瘤生长. |
| 英文摘要: |
| Objective To study the effects of internal exposure of 18F-FDG (18F-2-deoxy-2-fluoro-D-glucose) on the protein expressions of P53, XIAP (X-linked inhibitors of apoptosis protein) and GADD (growth arrest and DNA damage)45 in Lewis lung carcinoma, and to explore the possibility of applying 18F-FDG as a radiotherapy drug in vivo. Methods Lewis lung cancer transplanted tumor models were established via subcutaneous injection of 0.2 ml of 2×107/ml Lewis lung carcinoma cells at left hind limbs of 48 C57BL/6 mice that were randomly divided into high dose group, low dose group and control group with 16 mice each. After 7 d of cancer cell inoculation, 18.5×107 Bq and 9.25×107 Bq of 18F-FDG in 0.2 ml saline or equal volume of physiological saline was intraperitoneally injected into three group mice, respectively. 22 d post inoculation, the protein expressions of P53, XIAP and GADD45 were immuo-histochemically detected by using SP approach. Results There was significant difference among the protein expressions in each group (χ2=8.30, 16.02, 7.68, P<0.05). The mean integral optic density of protein expression increased from 0.089±0.033 in control group to 0.315±0.028 in high dose group for P53, and from 0.126±0.023 to 0.383±0.035 for GADD45, but it decreased from 0.422±0.034 to 0.149±0.055 for XIAP. There was significantly difference of these protein expressions in each group (P53:F=5.26,P<0.05;XIAP: F=4.29,P<0.05;GADD45:F=5.98,P<0.05). Conclusions 18F-FDG can up-regulate the expressions of P53 and GADD45 proteins and down-regulate the expression of XIAP protein in tumor cells, and it inhibits tumor growth by promoting cell apoptosis in the Lewis lung carcinoma tissue with a P53-dependent manner. |
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