| 何颖,沈先荣,王庆蓉,蒋定文,侯登勇,刘玉明,陈伟,李珂娴,刘琼,钱甜甜.低剂量γ射线照射对人淋巴母细胞蛋白表达的影响[J].中华放射医学与防护杂志,2014,34(11):832-836 |
| 低剂量γ射线照射对人淋巴母细胞蛋白表达的影响 |
| Effects of low dose γ-ray on protein expression of human lymphoblastoid cells |
| 投稿时间：2013-10-29 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.11.008 |
| 中文关键词: 低剂量 双向电泳 实时荧光定量PCR 真核翻译起始因子5A 脂肪酸结合蛋白4 |
| 英文关键词:Low-dose Two-dimensional electrophoresis Real-time PCR Eukaryotic translation initiation factor 5A Fatty acid binding protein 4 |
| 基金项目:全军计划科研项目(AHJ09J012);总后"十一五"科技攻关项目(2009183006) |
| 作者 | 单位 | E-mail | | 何颖 | 海军医学研究所, 上海 200433 | | | 沈先荣 | 海军医学研究所, 上海 200433 | xianrong_sh@163.com | | 王庆蓉 | 海军医学研究所, 上海 200433 | | | 蒋定文 | 海军医学研究所, 上海 200433 | | | 侯登勇 | 海军医学研究所, 上海 200433 | | | 刘玉明 | 海军医学研究所, 上海 200433 | | | 陈伟 | 海军医学研究所, 上海 200433 | | | 李珂娴 | 海军医学研究所, 上海 200433 | | | 刘琼 | 海军医学研究所, 上海 200433 | | | 钱甜甜 | 海军医学研究所, 上海 200433 | |
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| 中文摘要: |
| 目的 为探讨低剂量电离辐射生物效应作用机制,研究不同剂量单次照射后,人淋巴母细胞蛋白表达的变化.方法 利用双向凝胶电泳和基质辅助激光解析飞行时间串联质谱技术分离、鉴定不同低剂量 γ 射线导致的人淋巴母细胞差异表达蛋白,采用实时荧光定量PCR法从mRNA水平验证蛋白的差异表达.结果 不同低剂量 γ 射线照射后,双向电泳和质谱分离鉴定得到3个差异表达点:真核翻译起始因子5A(eIF5A),前折叠素亚单位1(PFDN1),脂肪酸结合蛋白4(FABP4).其中,eIF5A和FABP4的mRNA水平表达变化与双向电泳结果一致.结论 低剂量 γ 射线导致人淋巴母细胞中eIF5A、PFDN1和FABP4等蛋白表达发生变化,差异表达的蛋白有助于阐明低剂量辐射生物效应作用机制,并为进一步研究低剂量辐射暴露的生物标志物提供了参考. |
| 英文摘要: |
| Objective To observe the alterations of protein expression in human normal lymphoblastoid cell AHH-1 lines, after irradiation with different doses of γ-rays, in order to investigate mechanisms of biological effects induced by low dose radiation. Methods Two-dimensional electrophoresis (2-DE) was performed and differential expression proteins were identified by matrix assisted laser desorption/ionization time of flight mass spectrometry (MAIDI-TOF-MS) after irradiation. The mRNA expressions of proteins were analyzed by real-time quantity PCR (RTQ-PCR). Results Using 2-DE and MAIDI-TOF-MS, 3 kinds of altered proteins were definite in terms of eukaryotic translation initiation factor 5A (eIF5A), profoldin subunit 1(PFDN1) and fatty acid binding protein 4 (FABP4). The mRNA expressions of eIF5A and FABP4 were coincided with protein expressions. Conclusions These differentially expressed proteins induced by low-doses of γ-rays will help to elucidate the mechanisms of biological effects at low doses and offer the reference for further research of novel biomarkers for low dose exposure. |
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