| 袁晓丽,李涛,黄建鸣,查晓,邓碧芳,郞锦义.氯喹对食管癌细胞系放射增敏的实验研究[J].中华放射医学与防护杂志,2014,34(11):823-826 |
| 氯喹对食管癌细胞系放射增敏的实验研究 |
| Study of radiosensitization of chloroquine on esophageal cancer cell line |
| 投稿时间：2013-11-10 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.11.006 |
| 中文关键词: 食管肿瘤 自噬 氯喹 放射敏感性 |
| 英文关键词:Esophageal neoplasms Autophagy Chloroquine Radiosensitivity |
| 基金项目:四川省科技厅科技支撑项目(2012SZ0081) |
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| 中文摘要: |
| 目的 探讨氯喹对食管癌TE-1细胞系的放射增敏作用及其主要机制.方法 采用MTT法检测不同浓度氯喹对TE-1细胞的生长抑制作用.分别用单纯照射或照射前联合氯喹、照射后联合氯喹作用于TE-1细胞,作用6 h后用Western blot测定自噬相关蛋白LC3和Beclin1的表达,用Lyso-Tracker Red DND-99/Hochest 33258进行荧光染色,并用荧光显微镜观察细胞内酸性囊泡(AVOs)的变化.克隆形成实验分析细胞增殖的改变,拟合剂量-生存曲线并计算放射增敏参数.结果 氯喹对TE-1细胞的生长抑制作用呈浓度依赖性.辐射显著诱导TE-1细胞LC3和Beclin1的表达,并促进LC3-Ⅰ转换为LC3-Ⅱ.与单纯照射及照射前加药相比,照射后加药显著降低TE-1细胞内AVOs的荧光强度(F=16.44, P<0.05)和克隆形成率,照射前加药和照射后加药的SERD0分别为1.037和1.439(t=8.30, P<0.05).结论 氯喹能增加食管鳞癌TE-1细胞的放射敏感性,其机制可能与抑制自噬作用相关. |
| 英文摘要: |
| Objective To investigate the possibility of chloroquine radiosensitization of esophageal cancer cell line TE-1 and its further mechanism.Methods Effect of chloroquine on cell viability of TE-1 cells was determined by MTT method. Expression of LC3, Beclin-1 and formation of acidic vesicular organelles (AVOs) were determined by Western blot, and fluorescence staining with Lyso-Tracker Red DND-99, respectively. Clonogenic survival of TE-1 cells was examined by clonogenic forming assay. Results Chloroquine showed dose-dependent inhibition of TE-1 cell growth, and its values of IC50 and IC10 were (72.33±5.28) and (15.42±3.33) μmol/L, respectively. The expression of Beclin-1 and LC3-II/I markedly increased in irradiated TE-1 cells. The addition of chloroquine with IC10concentration significantly reduced the fluorescence and intensity of AVOs accumulation in the cytoplasm of TE-1 cells. Clonogenic survival fraction decreased obviously in TE-1 cells with addition of chloroquine after radiation and the value of SERD0 was 1.439. Conclusions Chloroquine could radiosensitize esophageal cancer cells by blocking autophagy-lysosomal pathway and be used as a potential radiosensitizing strategy. |
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