| 朱锦灿,陈小宇,刘成成,等.红景天苷对急性辐射损伤小鼠骨髓脂肪细胞生成的影响[J].中华放射医学与防护杂志,2014,34(10):735-738.Zhu Jincan,Chen Xiaoyu,Liu Chengcheng,et al.Effect of salidroside on radiation-induced bone marrow adipogenesis[J].Chin J Radiol Med Prot,2014,34(10):735-738 |
| 红景天苷对急性辐射损伤小鼠骨髓脂肪细胞生成的影响 |
| Effect of salidroside on radiation-induced bone marrow adipogenesis |
| 投稿时间：2014-03-18 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.10.004 |
| 中文关键词: 辐射 脂肪细胞 骨髓微环境 红景天苷 |
| 英文关键词:Radiation Adipocyte Bone marrow microenvironment Salidroside |
| 基金项目:国家自然科学基金（81270568） |
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| 摘要点击次数: 3805 |
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| 中文摘要: |
| 目的：探讨红景天苷抑制辐射诱导小鼠骨髓脂肪化、促进辐射后造血恢复的情况，并研究其可能的机制。方法：取6~7周龄健康BALB/c小鼠按随机数字表法分为健康对照组、单纯照射组、药物干预组，每组各20只。辐射对照组和实验组均给予6.0 Gy 60Co γ射线辐射处理，实验组于辐射后12 h腹腔注射红景天苷（30 mg·kg-1·d-1）至照后8 d，辐射对照组腹腔注射等体积生理盐水。辐射后14 d观察小鼠的一般情况、体重变化、外周血象，并取股骨制作骨髓切片观察骨髓病理改变，测定脂肪细胞面积，分离骨髓单个核细胞后提取总RNA，荧光定量PCR（q-PCR）检测PPAR-γ和FABP4 mRNA的相对表达量。结果：单纯照射组小鼠在照射后均出现外周血白细胞降低、血小板减低，骨髓脂肪细胞过度增生，骨髓有核细胞减少等骨髓造血抑制改变。与单纯照射组小鼠比较，红景天苷能改善照射后小鼠一般情况，并通过抑制PPAR-γ、FABP4的表达（t=8.64、13.19，P<0.05），抑制骨髓脂肪细胞的过度增生，减少脂肪空泡面积（t=13.31，P<0.05）；照后7 d，药物干预组小鼠外周血白细胞计数高于单纯照射组（t=5.80，P<0.05）；照后14 d，药物干预组小鼠外周血白细胞计数较单纯照射组及照后7 d药物干预组小鼠外周血白细胞计数均有增高（t=13.78、7.54，P<0.05），同时血红蛋白较单纯照射组高（t=14.66，P<0.05）。结论：红景天苷能抑制急性辐射损伤小鼠骨髓脂肪细胞生成，调节骨髓微环境，从而促进辐射损伤后小鼠造血恢复。 |
| 英文摘要: |
| Objective: To investigate the potential and underlying molecular mechanism of salidroside in ameliorating radiation-induced bone marrow adipogenesis and stimulating hematopoiesis. Methods: The female BALB/c mice aged 6-7 weeks were randomly divided into normal control group, radiation group and salidroside group. The radiation group and salidroside group were irradiated with 6.0 Gy of 60Co γ-rays. The salidroside group was intraperitoneally injected with 30 mg·kg-1·d-1 salidroside at 12 h and then every day until 8th d after radiation. The normal control group and radiation group were treated with equal volume of saline as control of salidroside. At 14 d after radiation, the mice weight, peripheral blood count, femur bone marrow histology, and the proportion of adipocyte area were measured, and the expressions of PPAR-γ and FABP4 were detected by q-PCR. Results: After irradiation, the numbers of white blood cells, hemoglobin and platelet in peripheral blood were reduced obviously, and the percentage of adipocyte area was increased significantly. Compared with mice in the radiation group, salidroside inhibited adipogenesis and reduced the proportion of adipocyte area (t=13.31, P<0.05) by reducing the expressions of PPAR-γ and FABP4 (t=8.64, 13.19, P<0.05). The number of white blood cells was partly recovered at 7 d after irradiation (t=5.80, P<0.05). Both white blood cells and hemoglobinin in peripheral blood of the salidroside group were higher than those in the radiation group at 14 d after irradiation. Conclusions: Salidroside could inhibit radiation-induced bone marrow adipogenesis and regulate bone marrow microenvironment, thereby promotes hematopoietic recovery in mice after radiation injury. |
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