| 常鹏宇,崔爽,罗景华,等.脂肪干细胞治疗放射性肠炎的实验研究[J].中华放射医学与防护杂志,2014,34(10):729-734.Chang Pengyu,Cui Shuang,Luo Jinghua,et al.Therapeutic effect of adipose-derived mesenchymal stem cells on radiation enteritis[J].Chin J Radiol Med Prot,2014,34(10):729-734 |
| 脂肪干细胞治疗放射性肠炎的实验研究 |
| Therapeutic effect of adipose-derived mesenchymal stem cells on radiation enteritis |
| 投稿时间：2014-03-31 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.10.003 |
| 中文关键词: 脂肪干细胞 放射性肠炎 细胞治疗 |
| 英文关键词:Adipose-derived mesenchymal stem cell Radiation enteritis Cell-based therapy |
| 基金项目:国家自然科学基金（81372929） |
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| 摘要点击次数: 3780 |
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| 中文摘要: |
| 目的：评价脂肪干细胞对放射性肠炎的治疗作用。方法：选用成年雄性SD大鼠，共52只。随机数字表法选取46只给予全腹15 Gy X射线照射制作放射性肠炎动物模型。造模结束后2 h，随机数字表法从受照大鼠中选取23只受照大鼠给予P6代青年女性脂肪干细胞腹腔注射治疗（Ad-MSC治疗组）；取另23只受照大鼠给予双磷酸盐缓冲液（PBS）腹腔注射治疗（PBS溶剂对照组）；剩余6只未照射大鼠作为健康对照组。造模后首个10 d内，分次抽取受照大鼠外周血，ELISA法检测血清中白介素-10（IL-10）的含量；分离受照大鼠胸腺细胞及外周血单个核细胞（PBMC），流式细胞术检测CD4/CD25/Foxp（3）阳性调节性T细胞的百分含量；获取受照小肠组织，HE染色分析炎症细胞浸润程度，Masson三色染色分析胶原成分的沉积程度，髓系过氧化物酶（MPO）免疫组织化学染色分析浸润的炎症细胞类型。Kaplan-Meier方法对受照大鼠进行生存分析。结果：在照射后30 d内，与PBS腹腔注射治疗的大鼠相比，接受脂肪干细胞治疗的大鼠的生存时间显著延长（t=4.53，P<0.05）。另外，脂肪干细胞能够上调受照大鼠外周血中IL-10的含量（7 d： t=13.93，P<0.05）；上调外周血（3.5 d：t=7.72，7 d：t=11.11，10 d：t=6.99，P<0.05）和胸腺（7 d：t=16.17，10 d：t=12.12，P<0.05）CD4/CD25/Foxp（3）阳性调节性T细胞的百分比；进而减轻炎症细胞在受照小肠中的浸润及胶原的沉积。结论：脂肪干细胞对放射性肠炎具有治疗作用。 |
| 英文摘要: |
| Objective: To evaluate the therapeutic effect of adipose-derived mesenchymal stem cells on radiation enteritis. Methods: A total of 52 male Sprague-Dawley rats were used in the present study. Herein, 46 rats were randomly selected and irradiated with a dose of 15 Gy at their abdomens. Two hours post-irradiation, 23 rats were randomly selected and infused intraperitoneally with adipose-derived mesenchymal stem cells in passage 6 from young-female donor. The other 23 rats were intraperitoneally infused with PBS. The rest 6 rats were set as normal control. During the first 10 days post-irradiation, peripheral blood-samples from irradiated rats were harvested for testing the levels of IL-10 in serum using ELISA assay. Additionally, after isolating the thymic cells and peripheral blood mononuclear cells, the percentages of CD4/CD25/Foxp(3)-positive regulatory T cells in thymus and peripheral blood were tested by flow-cytometry. Finally, infiltration of inflammatory cells and deposition of collagens within irradiated small intestine were analyzed by H&E staining and Masson Trichrome staining, respectively. Based on the MPO-immunohistochemistry staining, the type of infiltrated cells was identified. The Kaplan-Meier method was used for analyzing the survival rate of irradiated rats. Results: During a period of 30 days post-irradiation, the irradiated rats receiving adipose-derived mesenchymal stem cells survived longer than those receiving PBS (t=4.53, P<0.05). Compared to the irradiated rats with PBS-treatment, adipose-derived mesenchymal stem cells could elevate the level of IL-10 in serum (7 d: t=13.93, P<0.05) and increase the percentages of CD4/CD25/Foxp(3)-positive regulatory T cells in both peripheral blood (3.5 d: t=7.72, 7 d: t=11.11, 10 d: t=6.99, P<0.05) and thymus (7 d: t=16.17, 10 d: t=12.12, P<0.05). Moreover, infiltration of inflammatory cells and deposition of collagens within irradiated small intestine were mitigated by adipose-derived mesenchymal stem cells. Conclusions: Adipose-derived mesenchymal stem cells were capable of curing radiation enteritis. |
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