| 李瑾慧,刘军叶,蒋大鹏,苗霞,王亚峰,郭奇彦,郭国祯.电磁脉冲暴露对雄性BALB/c小鼠子代性别比的影响[J].中华放射医学与防护杂志,2014,34(8):588-591 |
| 电磁脉冲暴露对雄性BALB/c小鼠子代性别比的影响 |
| Effect of EMP on sex ratio of the offspring of male BALB/c mice |
| 投稿时间：2014-03-10 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.08.007 |
| 中文关键词: 电磁脉冲 睾丸指数 精子计数 子代性别比 |
| 英文关键词:Electromagnetic pulse Testis index Sperm number Offspring sex ratio |
| 基金项目:国家重点基础研究发展计划（973计划）（2011CB503704）；国家自然科学基金（51277175，81072272） |
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| 中文摘要: |
| 目的 探讨雄性BALB/c小鼠电磁脉冲（electromagnetic pulse，EMP）暴露对子代性别比的影响。方法 BALB/c小鼠雄性33只，雌性66只。雄性小鼠按照体重随机分为10 000次、100 000次脉冲辐照组和假辐照组。雌性小鼠相同环境分隔饲养，用以合笼。辐照组给予场强35 kV/m，重复频率50 Hz，脉冲数分别为10 000次和100 000次的辐照，每日1次，连续辐照2周。辐照后按雌雄比2：1合笼1周，1周后处死雄鼠，对比分析3组雄鼠的体重和睾丸重量，计算体睾指数；游离附睾中精子进行计数。孕鼠于孕20 d解剖取胎鼠组织进行PCR，鉴定子代性别比。结果与假辐照组相比，10 000次辐照组和100 000次辐照组雄鼠的体重、睾丸重量和体睾指数差异不大；精子计数均有下降，但差异无统计学意义。10 000次辐照组子代性别比（雄性：雌性）为0.298明显低于假辐照组0.871（χ2=9.37，P<0.01）；100 000次辐照组为0.826，与假辐照组相比差异无统计学意义。结论 雄性小鼠在场强35 kV/m，重复频率50 Hz，脉冲次数10 000次的EMP中暴露，可能导致子代雄性与雌性性别比降低。 |
| 英文摘要: |
| Objective To investigate the effects of EMP exposure to male BABL/c mice on the reproduction and its sex ratio. Methods Thirty-three male BALB/c mice were randomly divided into sham-exposed group, 10 000 pulses/d exposed group, and 100 000 pulses/d exposed group with 50 Hz static magnetic field of 35 kV/m for 2 weeks consecutively, then the male mice were mated with unexposed females (1 male vs. 2 female). Mated males were sacrificed to examine the exposure effects on sperm number and testis index after 7 days of mating. The sex ratio of embryos collected from unexposed mated females was investigated by PCR. Results The sex ratio of offspring was 0.298 (14/47) in the 10 000 pulses/d exposed group, significantly lower than 0.871 (27/31) in the sham-exposed group. No statistical changes were observed in the sex ratio of offspring, sperm number, the weights of testis and testis index after 100 000 pulses/d exposure. Conclusions The exposure of male mice to 35 kV/m EMP at 10 000 pulses/d for 2 weeks consecutively may change the sex ratio of mice's offspring. |
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