| 薛梦晨,蔡旭伟,刘琪,张琴,谢聪颖,傅小龙.辐射后玻连蛋白与胶原蛋白表达相关性的实验研究[J].中华放射医学与防护杂志,2014,34(8):573-577 |
| 辐射后玻连蛋白与胶原蛋白表达相关性的实验研究 |
| Experimental studies of the correlation between vitronectin and collagen expressions post-irradiation |
| 投稿时间：2013-10-28 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.08.004 |
| 中文关键词: 玻连蛋白 胶原蛋白 生物标记物 照射 成纤维细胞 |
| 英文关键词:Vitronectin Collagen Biological Markers Radiation Fibroblast |
| 基金项目:国家自然科学基金（81000993）；2010上海市卫生局青年科研项目（2010Y016） |
| 作者 | 单位 | E-mail | | 薛梦晨 | 200032 上海, 复旦大学附属肿瘤医院放疗科, 复旦大学上海医学院肿瘤学系 | | | 蔡旭伟 | 200032 上海, 复旦大学附属肿瘤医院放疗科, 复旦大学上海医学院肿瘤学系 | birdhome2000@163.com | | 刘琪 | 200032 上海, 复旦大学附属肿瘤医院放疗科, 复旦大学上海医学院肿瘤学系 | | | 张琴 | 200032 上海, 复旦大学附属肿瘤医院放疗科, 复旦大学上海医学院肿瘤学系 | | | 谢聪颖 | 温州医科大学附属第一医院放化疗科 | | | 傅小龙 | 200032 上海, 复旦大学附属肿瘤医院放疗科, 复旦大学上海医学院肿瘤学系 | |
|
| 摘要点击次数: 2847 |
| 全文下载次数: 1995 |
| 中文摘要: |
| 目的 分析不同剂量照射后成纤维细胞中玻连蛋白及胶原蛋白表达改变及其时间变化规律，初步探讨玻连蛋白作为放射性肺纤维化指标的意义。方法 采用137Cs辐射源，对人胚肺成纤维细胞WI-38、IMR-90各照射0、4、6、8、10和12 Gy，并于照后6、12、24、36、48和60 h收集细胞及培养上清液，采用Western blot法检测玻连蛋白和胶原蛋白Ⅰ、Ⅲ含量，采用实时PCR法及ELISA法分别检测玻连蛋白的转录水平及其细胞培养上清中的蛋白表达量。 结果 Western blot结果显示，受照射后两种成纤维细胞玻连蛋白及胶原蛋白Ⅰ、Ⅲ表达呈正相关性（r=0.40~0.79，P<0.05），都显著高于未经照射的对照组（t=3.04~25.45，P<0.05），且蛋白表达的剂量效应和时间效应均呈现先增高后降低的变化趋势。玻连蛋白与胶原蛋白在8~10 Gy照后48 h，表达量最高（t=2.92~18.86，P<0.05）。Real-time PCR结果与ELISA结果显示，玻连蛋白mRNA表达及其在上清中含量受照射剂量影响较显著（F=27.09~42.62，P<0.05），而受时间影响较小。 结论 照射后成纤维细胞中玻连蛋白在细胞内、上清中及mRNA表达水平均可能作为照射后纤维化的指标。而8 Gy照射及照射后48 h是产生成纤维细胞放射性纤维化的较优实验条件。 |
| 英文摘要: |
| Objective To analyze radiation induced alterations of vitronectin and collagen expressions in fibroblasts at different times post-irradiation, so as to evaluate the potential to apply vitronectin as a biomarker of radiation-induced lung fibrosis. Methods The human fibroblast cells WI-38 and IMR-90 were irradiated with 137Cs γ-rays at doses of 0 (control), 4, 6, 8, 10 and 12 Gy, respectively. The cells and its supernatant were collected at 6, 12, 24, 36, 48 and 60 h post-irradiation. The expressions of vitronectin and collagen Ⅰ and Ⅲ were analyzed by Western blot, PCR and ELISA. Results After irradiation, the expressions of vitronectin and collagen Ⅰ and Ⅲ were positively correlated (r=0.40-0.79, P<0.05) and were all significantly higher than that in control group (t=3.04-25.45,P<0.05) and reached the highest expression levels at 48 h after 8-10 Gy of irradiation (t=2.92-18.86,P<0.05). Analyses of Real-time PCR and ELISA assay showed that expressions of vitronectin mRNA and its protein level in the cell lysis were significantly increased by radiation (F=27.09-42.62, P<0.05). Conclusions The expressions of vitronectin in cellular supernatant and its mRNA may be a potential biomarker of radiation-induced fibrosis, and 48 h after 8 Gy irradiation may be an optimum condition of measurement. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|