| 卢宏达,董克臣,雷章,卢驰,陈卫群,孔庆志.连花清瘟对大鼠急性放射性肺损伤的防护作用[J].中华放射医学与防护杂志,2014,34(6):419-422 |
| 连花清瘟对大鼠急性放射性肺损伤的防护作用 |
| The protection of lianhuaqingwen against acute radiation-induced lung injury in rats |
| 投稿时间：2013-07-04 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.06.005 |
| 中文关键词: 急性放射性肺损伤 连花清瘟胶囊 肿瘤坏死因子-α 白介素-6 防护 |
| 英文关键词:Acute radiation-induced lung injury Lianhuaqingwen capsule Tumor necrosis factor-α (TNF-α) Interleukin-6 (IL-6) Protection |
| 基金项目:国家自然科学基金（81372931，81101550）；湖北省自然科学基金（2012FFB05904）；武汉市科技攻关计划（2013060602010253） |
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| 中文摘要: |
| 目的 探讨连花清瘟（Lianhuaqingwen，LHQW）对大鼠急性放射性肺损伤的防护作用及机制。方法 采用随机数字表法将36只雌性Wistar大鼠随机分为3组，给药组（照射+0.42g/kg组）、单纯照射组和空白对照组。除空白对照组，其他2组均以6 MV X射线右肺单次20 Gy照射制作大鼠急性肺损伤模型。给药组从照射前3 d开始至照射后第28天给予连花清瘟干膏粉溶液连续灌胃，空白对照组和单纯照射组灌生理盐水。照射后观察大鼠的一般情况，并于第1、14和28天处死大鼠观察肺病理切片，计数支气管肺泡灌洗液（BALF）中细胞总数，用ELISA法检测血清肿瘤坏死因子-α（TNF-α）和白介素-6（IL-6）水平，RT-PCR法检测肺组织中TNF-α和IL-6 mRNA的表达。结果 与单纯照射组比较，给药组的一般状况改善，病理形态学上，肺组织急性炎性反应明显减轻；给药组肺泡灌洗液细胞总数明显减少，治疗后14和28 d损伤肺组织中TNF-α和IL-6 mRNA表达均较单纯照射组低（tTNF-αmRNA=3.714、2.144，tIL-6 mRNA=3.589、2.883，P<0.05）；血清中TNF-α和IL-6水平较单纯照射组亦显著性降低（tTNF-α=7.372、2.891，tIL-6=6.335、3.257，P<0.05）。结论 连花清瘟能够改善急性放射性肺损伤大鼠的一般情况，减轻损伤肺组织的炎性反应，其机制与下调炎性因子TNF-α和IL-6的表达、抑制相关炎性介质的释放有关。 |
| 英文摘要: |
| Objective To investigate the radioprotective function of lianhuaqingwen (LHQW) in rat acute radiation-induced lung injury. Methods Totally 36 female Wistar rats were randomized into 3 groups as administered group (treated by LHQW plus radiation), radiation group irradiated with a single of 20 Gy in 6 MV X-ray by Elekta Synergy VMAT, and blank control group without radiation. Performance status (PS) was estimated during 31 d of LHQW instragastric administration. After rats being sacrificed at 1, 14, 28 d of LHQW adminstration, the pathomorphological changes were observed in trauma lung tissue, the cell number in BALF (Bronchoalveolar lavage fluid) was counted, the levels of TNF-α and IL-6 in serum were measured by ELISA, and TNF-α and IL-6 mRNA expressions in lung tissue were assayed by RT-PCR. Results After LHQW treatment, the PS of rat was significantly elevated with less inflammation in morphous, and the cell number in BALF was markedly decreased in compare with radiation alone group. Furthermore, the serum levels of TNF-α and IL-6 were obviously reduced (tTNF-α=7.372,2.891,tIL-6=6.335,3.257,P <0.05) and the TNF-α and IL-6 mRNA levels in lung tissue were also decreased (tTNF-αmRNA=3.714,2.144,tIL-6 mRNA=3.589,2.883,P<0.05). Conclusions LHQW plays a protective role against acute radiation-induced lung injury in rats and the down-expressions of TNF-α and IL-6 may be involved. |
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