| 俞辰逍,段卫明,周新文.LyGDI在非小细胞肺癌细胞A549中的辐射抗性机制研究[J].中华放射医学与防护杂志,2014,34(6):411-414 |
| LyGDI在非小细胞肺癌细胞A549中的辐射抗性机制研究 |
| Mechanism of the radioresistant effect of LyGDI on NSCLC A549 cells |
| 投稿时间：2013-07-12 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.06.003 |
| 中文关键词: LyGDI 非小细胞肺癌 A549细胞 辐射抗性 |
| 英文关键词:LyGDI Non-small-cell lung cancer A549 cell Radiation resistance |
| 基金项目:国家自然科学基金（30570548，81071878） |
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| 中文摘要: |
| 目的 探讨LyGDI的表达在非小细胞肺癌细胞A549中的辐射抗性机制。方法 A549和作为对照的H460细胞分别受到0、2、4和6 Gy X射线照射后，克隆形成试验分析辐射抗性差异，Western blot分析LyGDI和辐射敏感性关键基因环氧合酶COX-2的表达。实时荧光定量PCR分析miR-34a以及miR-34b/c在A549和H460细胞中的表达。A549细胞中转染50 nmol/L的miR-34c成熟序列，观察其对A549细胞辐射抗性以及LyGDI和COX-2基因表达的影响。结果 LyGDI和COX-2在辐射抗性的A549细胞中的表达水平高于H460细胞，miR-34a以及miR-34b/c在两种非小细胞肺癌中低表达。转染miR-34c可抑制LyGDI、COX-2和Bcl-2以及激活p21的表达，增强A549细胞细胞的辐射敏感性（t=3.85、5.89、5.12，P<0.05）。结论 LyGDI诱导的COX-2的上调表达以及电离辐射效应miR-34家族的下调表达，是A549细胞辐射抗性的一个主要原因。 |
| 英文摘要: |
| Objective To elucidate the mechanism of radiation resistant effect of LyGDI on NSCLC A549 cells. Methods A549 and H460 cells were irradiated with X-rays of 0, 2, 4 and 6 Gy. The clone-forming assay was used to detect cell survival and radiosensitivity. The expressions of LyGDI and COX-2 (Cyclooxygenase-2), a key radiosensitivity-related protein, were detected using Western blot. The miR-34 families were analyzed with RT-PCR. 50 nmol/L mature miR-34c was transfected into A549 cells. Results The expression levels of LyGDI and COX-2 were much higher in radioresistive A549 cells than that in H460 cells. While the expression of miR-34a was quite low and miR-34b/c was hardly found in both NSCLC cells. Transfection of miR-34c into A549 cells strongly enhanced X-ray induced apoptosis by inhibiting the activations of LyGDI, COX-2, Bcl-2 and p21. Conclusions Up-regulation of LyGDI could induce COX-2 expression. The low expression of miR-34 family might be responsible for the radiation resistance of NSCLC cells. |
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