| 常鹏宇,姜新,曲超,等.脂肪干细胞修复放射性肠上皮损伤的实验研究[J].中华放射医学与防护杂志,2014,34(4):271-274.Chang Pengyu,Jiang Xin,Qu Chao,et al.Therapeutic effects of adipose-derived stem cells on healing intestinal epithelium after abdominal irradiation[J].Chin J Radiol Med Prot,2014,34(4):271-274 |
| 脂肪干细胞修复放射性肠上皮损伤的实验研究 |
| Therapeutic effects of adipose-derived stem cells on healing intestinal epithelium after abdominal irradiation |
| 投稿时间：2013-07-12 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.04.008 |
| 中文关键词: 脂肪干细胞 放射性肠损伤 修复 |
| 英文关键词:Adipose-derived stem cells Radiation-induced intestinal injury Restoration |
| 基金项目:国家自然科学基金（81372929） |
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| 摘要点击次数: 3815 |
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| 中文摘要: |
| 目的 评价人源脂肪干细胞对放射性肠上皮损伤的修复作用。方法 选用成年雄性SD大鼠共40只，用随机数字表法从中选取34只给予腹部15 Gy X射线照射。造模结束后，取17只立即给予P6代人源脂肪干细胞腹腔注射治疗，移植的细胞数目为5×106/只；17只仅腹腔照射的大鼠作为对照；剩余6只大鼠作为健康对照组。于照后10 d，各组中抽签方法随机选取3只大鼠，并将其处死。取肠管进行HE染色、末端标记法（TUNEL）原位凋亡检测、Ki-67免疫组织化学染色以及Bmi1免疫组化染色；提取组织总RNA，实时荧光定量PCR检测受照肠组织表皮生长因子（EGF）和角质细胞生长因子（KGF），上皮细胞标记E-cadherin，凋亡相关基因Bcl-2及BAX的表达量。分析各组大鼠的生存时间及生存率。结果 与单纯照射组相比，人源脂肪干细胞（ADSC）可促进受照肠上皮细胞的增殖（t=18.30，P<0.05），抑制上皮细胞凋亡（t=-32.48，P<0.05），延长大鼠的生存时间（t=5.99，P<0.05），提高生存率。结论 人源脂肪干细胞对大鼠放射性肠上皮损伤具有修复作用。 |
| 英文摘要: |
| Objective To evaluate the therapeutic effect of human adipose-derived stem cells (ADSCs) on healing intestinal epithelium after abdominal irradiation. Methods A total of 40 Sprague-Dawley rats were irradiated to the whole abdomen with a single dose of 15 Gy. 17 rats were randomly selected and infused intraperitoneally with 5×106 Passage 6 ADSCs,and 17 rats that received abdominal irradiation alone were set as control, 6 rats were set as normal control. At 10 d post-irradiation, 3 rats from each group were randomly selected and terminated. Freshly isolated small intestinal tissues were used for pathological analysis and EGF, KGF, E-cadherin, Bcl-2 and BAX mRNA levels analysis, and life-span and survival of rats were also analyzed. Results Compared to the control, infusion of ADSCs maintained the integrity of irradiated epithelium with more proliferative epithelial cells(t=18.30,P<0.05)and less apoptotic cells(t=－32.48,P<0.05). Besides, the rats receiving ADSCs-infusion survived longer than the control(t=5.99,P<0.05). Conclusions ADSCs can have therapeutic effect on healing radiation-induced intestinal epithelium injury. |
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