| 赵骅,陆雪,陈德清,等.60Coγ射线诱导人外周血淋巴细胞核质桥的剂量-效应关系研究[J].中华放射医学与防护杂志,2014,34(4):259-262.Zhao Hua,Lu Xue,Chen Deqing,et al.Dose-effect of nucleoplasmic bridges frequencies in human peripheral blood lymphocytes induced by 60Co γ-rays[J].Chin J Radiol Med Prot,2014,34(4):259-262 |
| 60Coγ射线诱导人外周血淋巴细胞核质桥的剂量-效应关系研究 |
| Dose-effect of nucleoplasmic bridges frequencies in human peripheral blood lymphocytes induced by 60Co γ-rays |
| 投稿时间：2013-09-05 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.04.005 |
| 中文关键词: 电离辐射 核质桥 微核 剂量-效应 |
| 英文关键词:Ionizing radiation Nucleoplasmic bridge Micronucleus Dose response |
| 基金项目:国家自然科学基金（30870749，81172593） |
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| 中文摘要: |
| 目的 确定核质桥判定标准，建立 60Co γ 射线诱导正常人外周血淋巴细胞中核质桥（NPB）的剂量-效应曲线。 方法 用 60Co γ 射线照射3名健康男性离体外周血，照射剂量分别为0、1、2、3、4、5和6 Gy，剂量率为1 Gy/min，采用胞质分裂阻滞微核（CBMN）法进行细胞培养、收获、制片、染色。在光学显微镜下分析双核细胞中NPB及微核（MN）。 结果 在0~6 Gy 60Co γ 射线照射后，人外周血双核淋巴细胞中的NPB符合泊松分布，且NPB频率随吸收剂量增加而增加（H=19.51，P<0.01），拟合回归方程为线性平方模式y=（1.39×10-3）x2 + （4.94×10-3）x （R2=0.981，P < 0.01）。 结论 成功建立 0~6 Gy 60Co γ 射线诱导正常人外周血淋巴细胞中NPB的剂量-效应曲线。 |
| 英文摘要: |
| Objective To establish the analysis criteria of nucleoplasmic bridges (NPB) and a dose-response curve of NPB frequencies in human peripheral blood lymphocytes irradiated by 60Coγ-rays. Methods Human peripheral blood samples were collected from three healthy males, and were irradiated with 0,1, 2, 3, 4, 5 and 6 Gy of 60Coγ-rays. A cytokinesis-block micronucleus assay was carried out to analyze NPBs and micronuclei (MN) in binucleated cells. Results NPBs in binucleated cells at each dose level of γ-ray was conformed to the Poisson distribution. The dose-response curve of the γ-ray-induced NPB frequencies in human peripheral lymphocytes followed the linear-quadratic model y=(1.39×10-3)x2 + (4.94×10-3)x (R2=0.981, P < 0.05). Conclusions The dose-response curve of NPB frequencies in human peripheral blood lymphocytes induced by 0-6 Gy 60Coγ-rays was established. |
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