| 王金凤,孙新臣,康亚辉,王忠明,杨曦,蔡晶,张曲,何向锋,马建新,黄关宏.自由基清除剂Tempol对小鼠放射性下颌腺损伤防护机制的研究[J].中华放射医学与防护杂志,2014,34(3):176-179 |
| 自由基清除剂Tempol对小鼠放射性下颌腺损伤防护机制的研究 |
| Mechanism of the prevention effect of a free radical scavenger, Tempol, on radiation-induced dysfunction in the submandibular gland of mouse |
| 投稿时间：2013-06-09 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.03.005 |
| 中文关键词: 放射性损伤 自由基 氧化应激 下颌腺 |
| 英文关键词:Radiation injury Tempol Oxidative stress Submandibular gland |
| 基金项目: |
|
| 摘要点击次数: 3811 |
| 全文下载次数: 2544 |
| 中文摘要: |
| 目的 探讨不同浓度自由基清除剂Tempol（TPL）对C57bl/6小鼠下颌腺放射性损伤的防护作用及作用机制。方法 120只雄性C57bl/6小鼠，随机数字表法分为空白对照组、单纯照射组、25 mg/kg TPL组、200 mg/kg TPL、25 mg/kg TPL +照射和200 mg/kg TPL +照射组，共6个组，每组20只。两个联合治疗组在15 Gy照射前10 min腹腔注射给药。观察给药后30 d小鼠存活率、体重和照射后3和30 d小鼠唾液流率及下颌腺组织细胞凋亡及照射后3 d下颌腺组织总超氧化物歧化酶活性、总谷胱甘肽和丙二醛（MDA）含量及Bcl-2、Bax、Caspase-3蛋白表达水平。结果 照射后3和30 d单纯照射组小鼠唾液腺流率分别为5.67±1.05，5.27±1.34，与TPL 200 mg/kg+照射组比较，明显降低，差异有统计学意义（F=226.4，215.3，P<0.05），而与TPL25 mg/kg+照射组比较，差异无统计学意义；此外，TPL 200 mg/kg预处理降低照射后细胞的凋亡和组织MDA的含量（F=175.2，P<0.05），增加总谷胱甘肽（GSH）的含量和总超氧化物歧化酶（SOD）的活性（F=244.5、205.5，P<0.05），增加Bcl-2蛋白水平，降低Bax和活化Caspase-3蛋白水平。结论 200 mg/kg TPL对C57bl/6小鼠下颌腺组织具有放射保护作用，其机制可能为减轻组织氧化应激和调节线粒体凋亡信号通路，而25 mg/kg TPL没有此保护作用。 |
| 英文摘要: |
| Objective To explore the possible mechanism of the prevention effect of Tempol (TPL) on radiation-induced submandibular gland dysfunction using a C57bl/6 mouse model. Methods One-hundred twenty male C57bl/6 mice were randomly divided into six groups: blank control group, radiation group, 25 mg/kg TPL group, 200 mg/kg TPL group, 25 mg/kg TPL+ radiation group and 200 mg/kg TPL+ radiation group. 25 mg/kg and 200 mg/kg TPL was injected to mice enterocoelia 10 min before 15 Gy X-ray radiation. Survival rate and weight change of the mice were observed 30 d after radiation. Salivary flow rate and mandibular gland apoptosis were detected at 3 and 30 d after radiation. Total superoxide dismutase activity, total glutathione and malondialdehyde content and Bcl-2, Bax, Caspase 3 expression levels were detected at 3 d after radiation. Results Salivary flow rates at 3 and 30 days in the radiation group were 5.67±1.05 and 5.27±1.34, lower than that of 200 mg/kg TPL + radiation group (13.12±1.17,13.09±2.71) significantly (F=226.4, 215.3, P<0.05) but had no significant difference (P>0.05) compared with 25 mg/kg TPL + radiation group (6.44±1.09,5.48±1.79). Pretreatment of mice with 200 mg/kg TPL could reduce radiation induced apoptosis and the content of MDA in tissue but increased the GSH content. The TPL also increased the activity of SOD, the expression of Bcl-2 protein level, but reduced the levels of Bax and cleaved Caspase-3 protein. Conclusions 200 mg/kg TPL could protect C57bl/6 mice salivary glands from radiation injury probably by alleviating oxidative stress and regulating the mitochondrial pathway of apoptosis except 25 mg/kg TPL. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|