中华放射医学与防护杂志

王金凤,孙新臣,康亚辉,等.自由基清除剂Tempol对小鼠放射性下颌腺损伤防护机制的研究[J].中华放射医学与防护杂志,2014,34(3):176-179.Wang Jinfeng,Sun Xinchen,Kang Yahui,et al.Mechanism of the prevention effect of a free radical scavenger, Tempol, on radiation-induced dysfunction in the submandibular gland of mouse[J].Chin J Radiol Med Prot,2014,34(3):176-179

自由基清除剂Tempol对小鼠放射性下颌腺损伤防护机制的研究

Mechanism of the prevention effect of a free radical scavenger, Tempol, on radiation-induced dysfunction in the submandibular gland of mouse

投稿时间：2013-06-09

DOI：10.3760/cma.j.issn.0254-5098.2014.03.005

中文关键词: 放射性损伤自由基氧化应激下颌腺

英文关键词:Radiation injury Tempol Oxidative stress Submandibular gland

基金项目:

作者	单位	E-mail
王金凤	222000 连云港, 蚌埠医学院附属连云港第二人民医院放疗科
孙新臣	江苏省人民医院放疗科
康亚辉	222000 连云港, 蚌埠医学院附属连云港第二人民医院放疗科
王忠明	222000 连云港, 蚌埠医学院附属连云港第二人民医院放疗科
杨曦	江苏省人民医院放疗科
蔡晶	南通市肿瘤医院放化疗科
张曲	222000 连云港, 蚌埠医学院附属连云港第二人民医院放疗科
何向锋	南通市肿瘤医院放化疗科
马建新	222000 连云港, 蚌埠医学院附属连云港第二人民医院放疗科
黄关宏	222000 连云港, 蚌埠医学院附属连云港第二人民医院放疗科	15996587143@163.com

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中文摘要:

目的探讨不同浓度自由基清除剂Tempol（TPL）对C57bl/6小鼠下颌腺放射性损伤的防护作用及作用机制。方法 120只雄性C57bl/6小鼠，随机数字表法分为空白对照组、单纯照射组、25 mg/kg TPL组、200 mg/kg TPL、25 mg/kg TPL +照射和200 mg/kg TPL +照射组，共6个组，每组20只。两个联合治疗组在15 Gy照射前10 min腹腔注射给药。观察给药后30 d小鼠存活率、体重和照射后3和30 d小鼠唾液流率及下颌腺组织细胞凋亡及照射后3 d下颌腺组织总超氧化物歧化酶活性、总谷胱甘肽和丙二醛（MDA）含量及Bcl-2、Bax、Caspase-3蛋白表达水平。结果照射后3和30 d单纯照射组小鼠唾液腺流率分别为5.67±1.05，5.27±1.34，与TPL 200 mg/kg+照射组比较，明显降低，差异有统计学意义（F=226.4，215.3，P<0.05），而与TPL25 mg/kg+照射组比较，差异无统计学意义；此外，TPL 200 mg/kg预处理降低照射后细胞的凋亡和组织MDA的含量（F=175.2，P<0.05），增加总谷胱甘肽（GSH）的含量和总超氧化物歧化酶（SOD）的活性（F=244.5、205.5，P<0.05），增加Bcl-2蛋白水平，降低Bax和活化Caspase-3蛋白水平。结论 200 mg/kg TPL对C57bl/6小鼠下颌腺组织具有放射保护作用，其机制可能为减轻组织氧化应激和调节线粒体凋亡信号通路，而25 mg/kg TPL没有此保护作用。

英文摘要:

Objective To explore the possible mechanism of the prevention effect of Tempol (TPL) on radiation-induced submandibular gland dysfunction using a C57bl/6 mouse model. Methods One-hundred twenty male C57bl/6 mice were randomly divided into six groups: blank control group, radiation group, 25 mg/kg TPL group, 200 mg/kg TPL group, 25 mg/kg TPL+ radiation group and 200 mg/kg TPL+ radiation group. 25 mg/kg and 200 mg/kg TPL was injected to mice enterocoelia 10 min before 15 Gy X-ray radiation. Survival rate and weight change of the mice were observed 30 d after radiation. Salivary flow rate and mandibular gland apoptosis were detected at 3 and 30 d after radiation. Total superoxide dismutase activity, total glutathione and malondialdehyde content and Bcl-2, Bax, Caspase 3 expression levels were detected at 3 d after radiation. Results Salivary flow rates at 3 and 30 days in the radiation group were 5.67±1.05 and 5.27±1.34, lower than that of 200 mg/kg TPL + radiation group (13.12±1.17,13.09±2.71) significantly (F=226.4, 215.3, P<0.05) but had no significant difference (P>0.05) compared with 25 mg/kg TPL + radiation group (6.44±1.09,5.48±1.79). Pretreatment of mice with 200 mg/kg TPL could reduce radiation induced apoptosis and the content of MDA in tissue but increased the GSH content. The TPL also increased the activity of SOD, the expression of Bcl-2 protein level, but reduced the levels of Bax and cleaved Caspase-3 protein. Conclusions 200 mg/kg TPL could protect C57bl/6 mice salivary glands from radiation injury probably by alleviating oxidative stress and regulating the mitochondrial pathway of apoptosis except 25 mg/kg TPL.

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