| 孟大全,唐求,龙志雄,等.二甲苯基碘减轻放射性肺损伤作用的实验研究[J].中华放射医学与防护杂志,2014,34(3):168-171.Meng Daquan,Tang Qiu,Long Zhixiong,et al.Study of diphenylene iodonium in the prevention of radiation-induced lung injury[J].Chin J Radiol Med Prot,2014,34(3):168-171 |
| 二甲苯基碘减轻放射性肺损伤作用的实验研究 |
| Study of diphenylene iodonium in the prevention of radiation-induced lung injury |
| 投稿时间：2013-04-26 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.03.003 |
| 中文关键词: 二甲苯基碘 放射性肺损伤 大鼠 |
| 英文关键词:Diphenylene iodonium Radiation-induced lung injury Rats |
| 基金项目:国家自然科学基金（30770648） |
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| 中文摘要: |
| 目的 观察NADPH氧化酶抑制剂二甲苯基碘（DPI）对放射性肺损伤的防治作用，探讨其可能机制。方法 48只成年雄性SD大鼠按随机数字表法分为空白对照组（C组）、单纯照射组（R组）、照射+二甲苯基碘组（R+D组）和单用二甲苯基碘组（D组），每组12只。采用6 MV X射线照射双肺，每只每天照射8 Gy，连续照射5 d，总剂量40 Gy。R+D组大鼠在每次照射前1 h皮下注射0.02% DPI溶液（1 mg/kg）；D组大鼠不接受射线照射，只注射DPI溶液。两组大鼠DPI注射从照射前3 d开始，持续至照射后30 d。C组和R组大鼠则注射等体积的生理盐水。照射后1个月和6个月时提取肺组织，行HE染色、Masson染色、羟脯氨酸含量测定和TGF-β1免疫组织化学染色。结果 在照射后1个月时，R组出现典型肺泡炎表现，羟脯氨酸含量为（0.69±0.05）μg/mg，TGF-β1阳性染色面积比为（39.97±0.90）%，R+D组羟脯氨酸含量为（0.55±0.03）μg/mg，TGF-β1阳性染色面积比为（33.83±1.55）%，肺泡炎程度轻于R组（t=5.32、5.93，P<0.05）。6个月时R组出现典型肺纤维化表现，羟脯氨酸含量为（1.04±0.02）μg/mg，TGF-β1阳性染色面积比为（37.80±0.85）%，R+D组羟脯氨酸含量为（0.85±0.02）μg/mg，TGF-β1阳性染色面积比为（23.93±1.16）%，肺纤维化程度轻于R组（t=15.77、16.68，P<0.05）。C组和D组则未发生明显异常变化。结论 二甲苯基碘可以减少大鼠肺部照射后羟脯氨酸及TGF-β1的含量，减轻肺损伤的程度，对放射性肺损伤具有一定的防治作用。 |
| 英文摘要: |
| Objective To investigate the mechanism of an inhibitor of NADPH oxidases, diphenylene iodonium (DPI), in preventing radiation-induced lung injury. Methods Totally 48 adult SD male rats were randomly classified into 4 groups: control group (C), radiation group (R), radiation plus DPI group (R+D) and DPI group (D). The radiation induced pulmonary injury model was preformed by using 6 MV X-rays to deliver 8 Gy per day for 5 consecutive days with 40 Gy in total to the thorax of each animal. Rats in R+D group were subcutaneously administered with 0.02% DPI (1 mg/kg) at 1 h prior to radiation while rats in D group received the same dose of DPI without radiation. DPI was given from 3 d before radiation to 30 d after the first radiation. Rats in C and D groups received the same dose of saline. Animals were sacrificed at 1 month and 6 months after radiation, respectively. The lungs were removed and processed for HE and Masson staining, hydroxyproline content measurement, and TGF-β1 immunohistochemical detection.Results At 1 month post-radiation, rats in R group showed typical alveolitis, the level of hydroxyproline was (0.69±0.05) μg/mg, and the positive area of TGF-β1 expression was (39.97±0.90)%, while the level of hydroxyproline in R+D group was (0.55±0.03) μg/mg and the positive area of TGF-β1 expression was(33.83±1.55)%, rats in R+D group showed less severe alveolitis compared with R group(t=5.32,5.93,P<0.05). At 6 months post-radiation, rats in R group showed typical lung fibrosis with hydroxyproline level of (1.04±0.02) μg/mg and TGF-β1 expression of (37.80±0.85)%, whereas the hydroxyproline level in R+D group was (0.85±0.02) μg/mg, the TGF-β1 expression was(23.93±1.16)%, rats in R+D group showed moderate lung fibrosis(t=15.77,16.68,P<0.05), rats in C and D group had no noticeable changes.Conclusions Diphenylene iodonium could prevent radiation-induced lung injury by reducing the level of hydroxyproline and the expression of TGF-β1. |
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