| 王恺,马聪,邸婵娟,韩春光,李鹏飞,原美茹,刘超,刘永学.曲酸的抗氧化作用与辐射损伤防护效应的关系[J].中华放射医学与防护杂志,2014,34(2):83-87 |
| 曲酸的抗氧化作用与辐射损伤防护效应的关系 |
| Study on the relationship between the radioprotective effect and the anti-oxidation of kojic acid |
| 投稿时间：2013-06-06 |
| DOI：10.3760/cma.j.issn.0254-5098.2014.02.002 |
| 中文关键词: 曲酸 辐射防护 抗氧化 DPPH |
| 英文关键词:Kojic acid Radioprotection Antioxidation DPPH |
| 基金项目:国家科技重大专项资助课题（2011ZXJ0901-04C） |
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| 中文摘要: |
| 目的 探究曲酸的抗氧化作用与辐射防护效应的关系。方法 将C57小鼠按随机数字表法随机分为健康对照组、模型照射组、曲酸低浓度组及高浓度组。健康对照组不做任何处理，其他组在照前27 h分别皮下单次给予无菌蒸馏水、75及300 mg/kg的曲酸，经4 Gy 60Co γ射线单次全身照射，并于照后第3、4、7天处死小鼠，分别测定小鼠血清及肝肾组织中的总超氧化物歧化酶（T-SOD）活力、丙二醛（MDA）含量、股骨骨髓细胞DNA含量及骨髓细胞微核率，并体外测定曲酸对DPPH·自由基的清除作用。结果 曲酸300 mg/kg给药组小鼠血清及肝肾组织中总SOD活力、骨髓细胞DNA含量明显高于模型照射组（F=22.63、7.29、67.58、57.32，P<0.05），而其血清及肝肾组织中MDA含量、骨髓细胞微核率明显低于模型照射组（F=26.22、27.06、4.51、14.28，P<0.05），体外检测显示曲酸对DPPH·自由基具有明显的清除作用，曲酸、丁基羟基甲苯（BHT）清除DPPH·自由基的IC50值分别为（4.55±0.26）和（1.83±0.10）mg/ml，其浓度同为20 mg/ml时DPPH·自由基清除率分别为（82.38±3.51）%和（96.41±0.45）%。结论 曲酸可清除体内自由基，保护小鼠遗传物质免受射线损伤，从而实现其抗辐射作用。 |
| 英文摘要: |
| Objective To evaluate the relationship between the radioprotective effect and the anti-oxidation of kojic acid and provide experimental evidence for using kojic acid as a potential radioprotective medicine. Methods Male C57BL/6 mice were divided into 4 groups (normal group, irradiation group, low/high doses of kojic acid pretreated groups) by the random digits table method. Mice in normal group without any treatment, and mice in other groups were s.c. injected with a single dose of sterile distilled water, kojic acid (75 and 300 mg/kg, respectively) 27 h prior to a sublethal dose (4 Gy) of whole body gamma-irradiation. 20 mice were sacrificed at 3, 4, and 7 d post-irradiation, respectively. The activities of total superoxide dismutase (T-SOD) in serums and tissues (liver and kidney), the level of malondialdehyde (MDA), and the DNA contents and the frequency of micronuclei in femur bone marrow were assessed, and the radical-scavenging activity of kojic acid against stable DPPH· (2,2-diphenyl -1-picrylhydrazyl) was also determined. Results The activities of T-SOD in serums and tissues (liver and kidney) and the DNA content in bone marrow of mice pretreated with 300 mg/kg body weight of kojic acid were increased significantly (F= 22.63, 7.29, 67.58, 57.32, P<0.05) compared with the irradiation group, while the levels of MDA in serums and tissues (liver and kidney) and the frequency of micronuclei in bone marrow of mice pretreated with 300 mg/kg body weight of kojic acid decreased significantly (F= 26.22, 27.06, 4.51, 14.28, P<0.05) compared with the irradiation group. There was a marked radical-scavenging activity for kojic acid, and the IC50 values (which was defined as the concentration of kojic acid or BHT (2,6-di-tert-butyl-4 -methylphenol) causing 50% inhibition of absorbance) for kojic acid and BHT were (4.55±0.26)mg/ml and (1.83±0.10)mg/ml, respectively, and the results of DPPH· inhibition for kojic acid (20 mg/ml) and BHT (20 mg/ml) were (82.38±3.51)% and (96.41±0.45)%, respectively. Conclusions Kojic acid protects the hereditary substances of mice from radiation damage by scavenging free radicals in mice and exerts marked radioprotective effect. |
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