| 贾勇士,林白桦,毕爱红,詹文明,张丽萍,许丽萍,李强.肌肽对小鼠放射性肺损伤的防治作用[J].中华放射医学与防护杂志,2013,33(6):607-610 |
| 肌肽对小鼠放射性肺损伤的防治作用 |
| Prevention effect of carnosine on radiation-induced lung injury in mice |
| 投稿时间：2013-04-14 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.06.010 |
| 中文关键词: 肌肽 肺损伤 防治作用 转化生长因子-β1 |
| 英文关键词:Carnosine Lung injury Prevention TGF-β1 |
| 基金项目:浙江省医药卫生科技计划项目（2010KYA017） |
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| 中文摘要: |
| 目的 了解肌肽对小鼠放射性肺损伤的防治作用。方法 近交系C57/BL 雌性小鼠108只，随机数字表法分为对照组、单纯照射组、照射+肌肽组（15 mg·kg-1·d-1）和单纯肌肽组（15 mg·kg-1·d-1），对照组共18只，其他各组每组30只。采用10 MV X射线经前胸单次照射小鼠肺脏中平面13 Gy，全肺照射。两肌肽组灌胃给予15 mg·kg-1·d-1肌肽，照射前30 min给药1次，以后1 次/d至实验结束，对照组给予相应同体积生理盐水。在照射后7、28和56 d处死后取部分肺组织HE染色，部分肺组织行超氧化物歧化酶（SOD）检测，同时ELISA法测定血清中TGF-β1、TNF-α。结果 受照射组（单纯照射组、照射+肌肽组）在照射后56 d均表现出不同程度的放射性肺炎病理变化，照射+给药组较单纯照射组炎症反应明显较轻。各组间不同时段血清中TGF-β1水平差异有统计学意义（F=10.65、3.70、4.04，P<0.05）；各组间不同时段血清中TNF-α水平差异有统计学意义（F=39.55、53.38、4.01，P<0.05）；各组间不同时段肺组织中SOD水平差异有统计学意义（F=4.33、4.19、3.34，P<0.05）。照射+肌肽组较同期的单纯照射组，肺组织中SOD水平明显较高，血清中TGF-β1、TNF-α 明显降低。结论 肌肽通过保护SOD，降低TGF-β1和TNF-α水平起到预防并减轻小鼠放射性肺损伤的作用。 |
| 英文摘要: |
| Objective To investigate the protective effect of carnosine on radiation-induced lung injury in mice. Methods A total of 108 C57/BL female mice were randomly divided into 4 groups:control group without treatment, irradiation alone group, irradiation + carnosine group (15 mg·kg-1·d-1), and carnosine alone group (15 mg·kg-1·d-1). There were 18 mice in control group and 30 mice in every other group.Whole lung anterior chest was irradiated with a single dose of 13 Gy 10 MV X-rays. The mice were administered with carnosine (15 mg·kg-1·d-1) at 30 minutes before irradiation and then gavaged once a day until the end of the experiment.The control group was given with saline. At 7, 28, and 56 d after irradiation, 6 mice of control group and 10 mice of each other group were killed. A portion of lung tissues were stained with HE and other part of lung tissues were used to detect the levels of SOD. Meanwhile, TGF-β1 and TNF-α in the serum were detected with ELISA. Results Different levels of inflammation factors were expressed in the lung tissues of irradiation group and irradiation + carnosine group at 56 d after irradiation, but the inflammation in the irradiation + carnosine group was significantly lighter than that in the irradiation group. Among (7, 28 and 56 d) after radiation, TGF-β1, TNF-α, and SOD levels in different groups had significant differences. At the same time point after irradiation, the level of SOD in lung tissue of irradiation + carnosine group was significantly higher than that of irradiation group(F=4.33,4.19,3.34,P<0.05), but the levels of TGF-β1 and TNF-α in serum were reduced. ConclusionsCarnosine can prevent and inhibit radiation-induced lung injury in mice by increasing SOD and reducing TGF-β1 and TNF-α. |
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