| 陶华,郭业松,蒋鸣,王飞江.洛铂对鼻咽癌细胞CNE2体外放射增敏作用的研究[J].中华放射医学与防护杂志,2013,33(6):602-606 |
| 洛铂对鼻咽癌细胞CNE2体外放射增敏作用的研究 |
| Radiosensitization of lobaplatin on human nasopharyngeal cancer cell line CNE2 in vitro |
| 投稿时间：2013-01-17 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.06.009 |
| 中文关键词: 洛铂 鼻咽癌 CNE2细胞系 放射增敏 |
| 英文关键词:Lobaplatin Nasopharyngeal carcinoma CNE2 cell line Radiosensitivity |
| 基金项目: |
|
| 摘要点击次数: 2950 |
| 全文下载次数: 2165 |
| 中文摘要: |
| 目的 研究洛铂联合外照射对人鼻咽癌细胞系CNE2的杀伤和放射增敏作用及其机制。方法 以对数生长期的人鼻咽癌细胞系CNE2为研究对象，MTT法检测单纯洛铂和洛铂联合照射对CNE2细胞的增殖抑制作用；克隆形成试验分析洛铂对CNE2细胞的放射增敏作用；流式细胞仪检测单纯洛铂组和洛铂联合照射组细胞凋亡及细胞周期分布；蛋白免疫印迹检测Bcl-2、 Bax和Caspase-3蛋白表达。结果 洛铂对CNE2有增殖抑制作用，且细胞毒性呈剂量依赖性，IC50为1.610 μmol/L，洛铂联合照射60Co γ射线4 Gy对CNE2细胞IC50为0.077 μmol/L。洛铂联合照射组放射增敏比大于3。24 h内随洛铂浓度增加，鼻咽癌细胞进入G2/M期比例增多。而洛铂联合照射将鼻咽癌细胞更多地阻滞于G2/M期，当洛铂浓度增大到6 μmol/L，洛铂联合照射组主要将细胞阻滞于S期。洛铂5 μmol/L作用24 h可引起15.6%的细胞凋亡，4 Gy照射可引起11.3%细胞凋亡，洛铂联合照射组可引起61.8%的细胞凋亡。蛋白免疫印迹结果显示，洛铂联合照射组抑制凋亡蛋白Bcl-2表达水平下降，促进凋亡诱导蛋白Bax以及活化的Caspase-3表达水平升高。结论 洛铂对人鼻咽癌CNE2细胞有放射增敏作用，作用机制可能与抑制Bcl-2表达，促进Bax表达，激活Bcl-2（Bax）-Caspase信号通路有关。 |
| 英文摘要: |
| Objective To investigate the effect of lobaplatin combined with irradiation on human nasopharyngeal cancer cell line CNE2, and to illuminate its mechanism of radiosensitization. Methods MTT assay was used to detect the outcome of lobaplatin and irradiation on CNE2 cell proliferation. Clonogenic assay was applied to testify the radiosensitization effect of lobaplatin on the cells. Flow cytometry was used to check the cell cycle distribution and cell apoptosis. Western was used to detect the expression of Bcl-2, Bax and cleaved Caspase-3. Results The proliferation of CNE2 cells was reduced by lobaplatin in a dose-dependent manner. IC50 of lobaplatin on CNE2 cells and lobaplatin combined with 4 Gy irradiation was 1.610 μmol/L and 0.077 μmol/L, respectively. The radiosensitization ratio of the combination group was over 3. Within 24 h of drug treatment,the percent of cells in G2/M phase increased with the concentration of lobaplatin. When the concentration of lobaplatin increased to 6 μmol/L, the cells of combination group were arrested at S phase. The apoptosis rate of lobaplatin(5 μmol/L)group,radiotherapy(4 Gy)group and combination group was 15.6%, 11.3% and 61.8%,respectively. Western blot showed that the expressions of Bax and cleaved Caspase-3 increased but Bcl-2 decreased in the combination group. Conclusion Lobaplatin could increase radiosensitization of human nasopharyngeal cancer cell line CNE2, probably by depressing Bcl-2 but enhancing Bax expression and hence activating Bcl-2/Bax-Caspase signaling pathway. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |