| 王慧星,霍小东,史可梅,郑宝森,刘静芷,张文艺,焦玲,杨景魁,马文庭.125I放射性粒子组织间植入近距离照射大鼠背根神经节的研究[J].中华放射医学与防护杂志,2013,33(6):588-592 |
| 125I放射性粒子组织间植入近距离照射大鼠背根神经节的研究 |
| Study on the rat’s dorsal root ganglion after 125I brachytherapy |
| 投稿时间：2012-11-25 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.06.005 |
| 中文关键词: 125I粒子 近距离治疗 背根神经节 损伤 |
| 英文关键词:125I seeds Brachytherapy Dosal root ganglion Injury |
| 基金项目: |
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| 摘要点击次数: 3294 |
| 全文下载次数: 1974 |
| 中文摘要: |
| 目的 观察植入不同活度放射性125I粒子近距离照射后，大鼠后肢行为学和背根神经节（DRG）放射损伤的超微结构变化。方法 SD大鼠36只，采用随机数字表法随机分为3组：空白组（钛质空壳作为对照）和低、高活度放射性125I粒子组。低、高活度组分别于L4~5、L5~6椎间孔植入1粒放射活度为14.8和29.6 MBq 125I粒子。3组分别于粒子植入术前24 h、术后48、168、336、720和1440 h，用vonFrey hair测量手术前后大鼠后肢50%的机械刺激撤足阈值（PWT），运用联合行为评分（CBS）对大鼠的后肢运动情况进行评估，于术后336和1440 h，每组取2只大鼠L5的DRG经电镜观察超微病理学变化。结果 与术前进行比较，高活度组术后1440 h后肢50%的机械刺激撤足阈值明显升高（t=2.972，P<0.05），CBS评分差异无统计学意义；低活度组术后336 h时DRG内大部分为正常形态的节细胞，少量异常细胞。低活度组术后1440 h和高活度组术后336 h背根神经节细胞核膜有皱褶，核仁边集，线粒体普遍肿胀，空泡变明显，溶酶体增多，部分内质网肿胀。高活度组术后1440 h时大量节细胞出现核膜皱缩，部分模糊，大部分空泡变，溶酶体明显增多，内质网肿胀扩张等细胞坏死表现。神经纤维的髓鞘皱缩，线粒体少，空泡样变化明显。未见细胞核固缩、凋亡小体等细胞凋亡迹象。结论 放射性125I粒子近距离照射可引起大鼠背根神经节超微结构损伤，引起后肢痛阈升高，对后肢运动无影响。 |
| 英文摘要: |
| Objective To observe the changes of the ultrastructure of dorsal root ganglion (DRG) and the behaviors of hind limb after implantation of radioactive 125I seeds around the DRG in rats. Methods 36 adult male Sprague-Dawley rats were randomly divided into three groups with different radioactivity 125I seeds implanted around DRG, named control group (no irradiation, n=12), low radioactivity group (14.8 MBq, n=12) and high radioactivity group (29.6 MBq, n=12). The paw withdrawal threshold and combined behavior score were observed 24 h prior to seed implantation and at 48, 168, 336, 720 and 1440 h after the treatment. Two rats were killed at 336 h and 1440 h to observe the ultrastructure of L5 DRG. Results Compared to the control without operation, PWT of the high radioactivity group were significantly higher than that of other groups (t=2.972,P<0.05). The in most of DRG low radioactivity group at 336 h were normal with a few abnormal cells. The DRG at 1440 h in low radioactivity group and in at 336 h high radioactivity group showed nuclear membrane buckling, nucleolus-set, mitochondrial swelling, lysosome increase, endoplasmic reticulum dilation and vacuolization. At 1440 h in the high radioactivity group, most of nuclear membrane invaginated, part of nuclear membrane obscured and mitochondrial swelled, but nuclear pyknosis and apoptotic body were not observed. Conclusions DRG can be injured after implantation of radioactive 125I around DRG, and the threshold of mechanical pain would be elevated after implanting radioactive 125I seeds. |
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