| 李晓,江其生,李峰生,等.低剂量X射线对人树突状细胞体外迁移能力的影响及其机制[J].中华放射医学与防护杂志,2013,33(5):476-479.LI Xiao,JIANG Qi-sheng,LI Feng-sheng,et al.Effect of low dose X-ray irradiation on migration of human dendrite cells and underlying mechanism[J].Chin J Radiol Med Prot,2013,33(5):476-479 |
| 低剂量X射线对人树突状细胞体外迁移能力的影响及其机制 |
| Effect of low dose X-ray irradiation on migration of human dendrite cells and underlying mechanism |
| 投稿时间：2013-01-18 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.005 |
| 中文关键词: X射线 树突状细胞 迁移 CCR7 |
| 英文关键词:Irradiation Dendritic cells Migration CCR7 |
| 基金项目:国家自然科学基金(81172130/H1610);国家自然科学基金青年项目(81202151) |
| 作者 | 单位 | E-mail | | 李晓 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | | | 江其生 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | jqs598@sina.com | | 李峰生 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | | | 吕进 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | | | 王思念 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | | | 何蕊 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | | | 邹跃 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | | | 孙松韦 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | | | 王成 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | | | 刘家赵 | 100088 北京, 第二炮兵总医院 全军核辐射损伤医学监测与防治研究中心 | |
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| 中文摘要: |
| 目的 研究低剂量X射线对人树突状细胞(DC)体外迁移能力影响并探讨其机制。方法 分离人外周血单个核细胞(PBMC),以人GM-CSF和IL-4共同诱导分化为DC,于培养的第5天加入TNF-α促进成熟,在培养的第6天用X射线照射DC,受照剂量分别是0、0.05、0.1、0.2、0.5 Gy,照射后48 h收获DC;采用RT-PCR法及Western blot法分别检测CCR7 mRNA及蛋白表达水平;Transwell迁移实验法检测DC的体外迁移能力。结果 与0 Gy相比,0.2和0.5 Gy照射后,CCR7 mRNA的相对表达量显著高于其他剂量(t=14.72、4.72,P<0.05);0.2 Gy照射后,CCR7蛋白表达量高于其他剂量(t=4.46,P<0.05),DC迁移能力显著高于其他剂量(t=2.95,P<0.05);以抗CCR7单克隆抗体封闭CCR7蛋白活性,在接受同等剂量照射时,DC细胞迁移能力显著降低(t=4.63~8.96,P<0.05)。结论 受到0.2 Gy X射线照射的DC,体外迁移能力显著增强,其机制可能与受照后DC表达CCR7 mRNA及蛋白水平升高有关。 |
| 英文摘要: |
| Objective To study the effect of low dose X-ray irradiation on the migration of human dendritic cells and its mechanism in vitro. Methods The human peripheral blood mononuclear cells (PBMC) were separated and treated with rhGM-CSF and rhIL-4 in order to differentiate them to dendritic cells (DC) in vitro. To enhance cell maturation, 50 mg/L TNF-α was added into the medium at 5 d of culture. At 6 d of cell culture, DCs were radiated with X-rays of 0.05, 0.1, 0.2 and 0.5 Gy, respectively. At 8 d, the dendrite cells were collected for further analysis. The expressions of CC-Chemokine Receptor 7 (CCR7) mRNA and its protein were detected by RT-PCR and Western blot, respectively. Transwell culture inserts were used to measure the amount of migrated cells. Results After 0.2 and 0.5 Gy radiation, the expression of CCR7 mRNA of DCs was remarkably increased(t=14.72, 4.72,P<0.05), but the expression of CCR7 protein in the DCs irradiated with 0.2 Gy was higher than that irradiated with other doses(t=4.46, P<0.05), meanwhile the amount of migrated DCs was obviously increased(t=2.95, P<0.05). Furthermore, DCs treated with anti-CCR7 monoclone antibody decreased the ability of radiation-induced migration(t=4.63-8.96,P<0.05). Conclusions 0.2 Gy X-ray irradiation significantly can enhance the migration ability of DCs in vitro, which may be correlated with the increase of CCR7 expression. |
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