| 李峰生,高玲,宋秀军,李晓,江其生,陈肖华.电离辐射对人肺腺癌A549细胞中STAT3靶基因表达的影响[J].中华放射医学与防护杂志,2013,33(5):472-475 |
| 电离辐射对人肺腺癌A549细胞中STAT3靶基因表达的影响 |
| Effect of ionizing radiation on the expressions of STAT3 target genes in A549 cells |
| 投稿时间：2013-05-16 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.004 |
| 中文关键词: 电离辐射 STAT3 血管内皮细胞生长因子 存活素 |
| 英文关键词:Ionizing radiation STAT3 VEGF Survivin |
| 基金项目:国家自然科学基金(8120215,81172130,81001216) |
|
| 摘要点击次数: 3335 |
| 全文下载次数: 2354 |
| 中文摘要: |
| 目的 探讨γ射线照射对人肺腺癌A549细胞中促血管生成基因VEGF以及抗凋亡基因bcl-2和存活素(survivin)表达的影响及其与转录因子STAT3的关系。方法 利用RT-PCR和Western blot检测2和4 Gy γ射线照射后24 h A549细胞中VEGF、Bcl-2和Survivin表达水平以及STAT3抑制后VEGF和Survivin的表达变化。结果 2和4 Gy γ射线照射后,VEGF和Survivin的表达均显著升高,而Bcl-2的表达不受γ射线照射的影响。利用抑制剂AG490阻断γ射线照射诱导的STAT3激活后,辐射诱导的VEGF和Survivin的表达升高被抑制。结论 γ射线照射可通过激活STAT3上调A549细胞中Survivin和VEGF的表达,Survivin蛋白的上调在一定程度上抑制了辐射诱导的肿瘤细胞凋亡,而VEGF的表达升高则有助于肿瘤血管生成。 |
| 英文摘要: |
| Objective To investigate the effect of ionizing radiation on the expression of transcription factor STAT3 target genes in the pulmonary adenocarcinoma cell line A549. Methods The expressions of VEGF, Bcl-2 and Survivin in A549 cells with or without STAT3 inhibition were detected by RT-PCR and/or Western blot 24 h after 2 or 4 Gy of γ-ray irradiation. Results After γ-ray irradiation with 2 or 4 Gy, the expression of VEGF and Survivin increased significantly. However, the expression of Bcl-2 was not affected by γ-ray irradiation. Furthermore, the increased expression of VEGF and Survivin induced by radiation was found to be inhibited after radiation-induced activation of STAT3 was blocked by AG490 inhibiter. Conclusions γ-ray irradiation could up-regulate the expression of Survivin and VEGF via activating STAT3. The increase of Survivin might partly inhibit the radiation-induced apoptosis of A549 cells, and the up-regulation of VEGF might contribute to the tumor angiogenesis. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |