| 周慧,杨冰,唐泉,孙元明,韩英,樊飞跃,贾立立.电离辐射对成骨细胞核因子κB受体活化因子配体和骨保护素mRNA及其蛋白表达的影响[J].中华放射医学与防护杂志,2013,33(5):468-471 |
| 电离辐射对成骨细胞核因子κB受体活化因子配体和骨保护素mRNA及其蛋白表达的影响 |
| Effect of ionizing radiation on the expressions of RANKL and OPG mRNA and proteins in osteoblasts |
| 投稿时间：2013-04-08 |
| DOI：10.3760/cma.j.issn.0254-5098.2013.05.003 |
| 中文关键词: 电离辐射 成骨细胞 破骨细胞 核因子κB受体活化因子配体 骨保护素 |
| 英文关键词:Ionizing radiation Osteoblasts Osteoclasts Receptor activator for NF-κB ligand Osteoprotegerin |
| 基金项目:国家自然科学基金(30970867) |
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| 中文摘要: |
| 目的 研究电离辐射对成骨细胞RANKL和OPG表达的影响,探讨辐射导致骨损伤的分子机制。方法 采用MC3T3-E1细胞诱导分化为成骨细胞,经0、2和4 Gy137Cs γ射线照射后,采用实时定量PCR及Western blot方法检测电离辐射对于成骨细胞RANKL和OPG mRNA及其蛋白水平表达的影响。结果 4 Gy照射可以导致成骨细胞RANKL mRNA(t=5.41,P<0.05)及其蛋白(t=68.37,P<0.01)表达水平上调;2和4 Gy照射可以导致成骨细胞OPG mRNA(t=5.20、7.02,P<0.05)及其蛋白(t=7.78、9.45,P<0.05)表达水平下调。结论 2和4 Gy电离辐射可以导致成骨细胞中RANKL/RANK/OPG通路发生改变,促进破骨细胞的分化和成熟,进而促进破骨细胞的骨吸收作用。 |
| 英文摘要: |
| Objective To investigate the effect of radiation on the expressions of RANKL and OPG in osteoblasts in order to disclose the molecular mechanism of bone injury induced by ionizing radiation. Methods The osteoblasts were differentiated from MC3T3-E1 cells. After 2 or 4 Gy137Cs γ-irradiation, the mRNA and protein expression levels of RANKL and OPG of osteoblast precursor and osteoblast were detected by real-time PCR and Western blot. Results The expressions of RANKL mRNA (t=5.41, P<0.05) and protein (t=68.37, P<0.01) were up-regulated after 4 Gy irradiation, while the expressions of OPG mRNA (t=5.20,7.02,P<0.05) and protein (t=7.78,9.45,P<0.05) were down-regulated after 2 and 4 Gy irradiation. Conclusions 2 and 4 Gy ionizing radiation alters RANKL/RANK/OPG pathway in osteoblasts, which may promote the osteoclast differentiation and maturation and hence promote bone resorption of osteoclasts. |
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